Frontiers in Cardiovascular Medicine (Oct 2022)
Anisotropic topographies restore endothelial monolayer integrity and promote the proliferation of senescent endothelial cells
- Vasileios Exarchos,
- Vasileios Exarchos,
- Sebastian Neuber,
- Sebastian Neuber,
- Heike Meyborg,
- Heike Meyborg,
- Costanza Giampietro,
- Costanza Giampietro,
- Nafsika Chala,
- Silvia Moimas,
- Hristian Hinkov,
- Hristian Hinkov,
- Friedrich Kaufmann,
- Francesca M. Pramotton,
- Francesca M. Pramotton,
- Katrin Krüger,
- Katrin Krüger,
- Katrin Krüger,
- Hector Rodriguez Cetina Biefer,
- Hector Rodriguez Cetina Biefer,
- Hector Rodriguez Cetina Biefer,
- Nikola Cesarovic,
- Nikola Cesarovic,
- Dimos Poulikakos,
- Volkmar Falk,
- Volkmar Falk,
- Volkmar Falk,
- Maximilian Y. Emmert,
- Maximilian Y. Emmert,
- Maximilian Y. Emmert,
- Maximilian Y. Emmert,
- Maximilian Y. Emmert,
- Aldo Ferrari,
- Aldo Ferrari,
- Aldo Ferrari,
- Timo Z. Nazari-Shafti,
- Timo Z. Nazari-Shafti,
- Timo Z. Nazari-Shafti
Affiliations
- Vasileios Exarchos
- Cardiosurgical Research Group, Department of Cardiothoracic and Vascular Surgery, German Heart Center Berlin, Berlin, Germany
- Vasileios Exarchos
- Translational Cardiovascular Regenerative Technologies Group, BIH Center for Regenerative Therapies, Berlin Institute of Health at Charité—Universitätsmedizin Berlin, Berlin, Germany
- Sebastian Neuber
- Cardiosurgical Research Group, Department of Cardiothoracic and Vascular Surgery, German Heart Center Berlin, Berlin, Germany
- Sebastian Neuber
- Translational Cardiovascular Regenerative Technologies Group, BIH Center for Regenerative Therapies, Berlin Institute of Health at Charité—Universitätsmedizin Berlin, Berlin, Germany
- Heike Meyborg
- Cardiosurgical Research Group, Department of Cardiothoracic and Vascular Surgery, German Heart Center Berlin, Berlin, Germany
- Heike Meyborg
- Translational Cardiovascular Regenerative Technologies Group, BIH Center for Regenerative Therapies, Berlin Institute of Health at Charité—Universitätsmedizin Berlin, Berlin, Germany
- Costanza Giampietro
- Experimental Continuum Mechanics, Empa Swiss Federal Laboratories for Materials Science and Technology, Dübendorf, Switzerland
- Costanza Giampietro
- Department of Mechanical and Process Engineering, Institute for Mechanical Systems, ETH Zürich, Zurich, Switzerland
- Nafsika Chala
- Laboratory of Thermodynamics in Emerging Technologies, Department of Mechanical and Process Engineering, ETH Zürich, Zurich, Switzerland
- Silvia Moimas
- Laboratory of Thermodynamics in Emerging Technologies, Department of Mechanical and Process Engineering, ETH Zürich, Zurich, Switzerland
- Hristian Hinkov
- Cardiosurgical Research Group, Department of Cardiothoracic and Vascular Surgery, German Heart Center Berlin, Berlin, Germany
- Hristian Hinkov
- Translational Cardiovascular Regenerative Technologies Group, BIH Center for Regenerative Therapies, Berlin Institute of Health at Charité—Universitätsmedizin Berlin, Berlin, Germany
- Friedrich Kaufmann
- Cardiosurgical Research Group, Department of Cardiothoracic and Vascular Surgery, German Heart Center Berlin, Berlin, Germany
- Francesca M. Pramotton
- Experimental Continuum Mechanics, Empa Swiss Federal Laboratories for Materials Science and Technology, Dübendorf, Switzerland
- Francesca M. Pramotton
- Department of Mechanical and Process Engineering, Institute for Mechanical Systems, ETH Zürich, Zurich, Switzerland
- Katrin Krüger
- Cardiosurgical Research Group, Department of Cardiothoracic and Vascular Surgery, German Heart Center Berlin, Berlin, Germany
- Katrin Krüger
- Translational Cardiovascular Regenerative Technologies Group, BIH Center for Regenerative Therapies, Berlin Institute of Health at Charité—Universitätsmedizin Berlin, Berlin, Germany
- Katrin Krüger
- Clinic for Cardiovascular Surgery, Charité—Universitätsmedizin Berlin, Berlin, Germany
- Hector Rodriguez Cetina Biefer
- Cardiosurgical Research Group, Department of Cardiothoracic and Vascular Surgery, German Heart Center Berlin, Berlin, Germany
- Hector Rodriguez Cetina Biefer
- Translational Cardiovascular Regenerative Technologies Group, BIH Center for Regenerative Therapies, Berlin Institute of Health at Charité—Universitätsmedizin Berlin, Berlin, Germany
- Hector Rodriguez Cetina Biefer
- Department of Cardiac Surgery, City Hospital of Zürich, Site Triemli, Zurich, Switzerland
- Nikola Cesarovic
- Cardiosurgical Research Group, Department of Cardiothoracic and Vascular Surgery, German Heart Center Berlin, Berlin, Germany
- Nikola Cesarovic
- Department of Health Sciences and Technology, ETH Zürich, Zurich, Switzerland
- Dimos Poulikakos
- Laboratory of Thermodynamics in Emerging Technologies, Department of Mechanical and Process Engineering, ETH Zürich, Zurich, Switzerland
- Volkmar Falk
- Clinic for Cardiovascular Surgery, Charité—Universitätsmedizin Berlin, Berlin, Germany
- Volkmar Falk
- Department of Health Sciences and Technology, ETH Zürich, Zurich, Switzerland
- Volkmar Falk
- Department for Cardiovascular and Thoracic Surgery, German Heart Center Berlin, Berlin, Germany
- Maximilian Y. Emmert
- Cardiosurgical Research Group, Department of Cardiothoracic and Vascular Surgery, German Heart Center Berlin, Berlin, Germany
- Maximilian Y. Emmert
- Translational Cardiovascular Regenerative Technologies Group, BIH Center for Regenerative Therapies, Berlin Institute of Health at Charité—Universitätsmedizin Berlin, Berlin, Germany
- Maximilian Y. Emmert
- Clinic for Cardiovascular Surgery, Charité—Universitätsmedizin Berlin, Berlin, Germany
- Maximilian Y. Emmert
- Department of Health Sciences and Technology, ETH Zürich, Zurich, Switzerland
- Maximilian Y. Emmert
- 0Institute for Regenerative Medicine, University of Zurich, Zurich, Switzerland
- Aldo Ferrari
- Experimental Continuum Mechanics, Empa Swiss Federal Laboratories for Materials Science and Technology, Dübendorf, Switzerland
- Aldo Ferrari
- Department of Mechanical and Process Engineering, Institute for Mechanical Systems, ETH Zürich, Zurich, Switzerland
- Aldo Ferrari
- Laboratory of Thermodynamics in Emerging Technologies, Department of Mechanical and Process Engineering, ETH Zürich, Zurich, Switzerland
- Timo Z. Nazari-Shafti
- Cardiosurgical Research Group, Department of Cardiothoracic and Vascular Surgery, German Heart Center Berlin, Berlin, Germany
- Timo Z. Nazari-Shafti
- Translational Cardiovascular Regenerative Technologies Group, BIH Center for Regenerative Therapies, Berlin Institute of Health at Charité—Universitätsmedizin Berlin, Berlin, Germany
- Timo Z. Nazari-Shafti
- 1BIH Biomedical Innovation Academy, BIH Charité (Junior) (Digital) Clinician Scientist Program, Berlin Institute of Health at Charité—Universitätsmedizin Berlin, Berlin, Germany
- DOI
- https://doi.org/10.3389/fcvm.2022.953582
- Journal volume & issue
-
Vol. 9
Abstract
Thrombogenicity remains a major issue in cardiovascular implants (CVIs). Complete surficial coverage of CVIs by a monolayer of endothelial cells (ECs) prior to implantation represents a promising strategy but is hampered by the overall logistical complexity and the high number of cells required. Consequently, extensive cell expansion is necessary, which may eventually lead to replicative senescence. Considering that micro-structured surfaces with anisotropic topography may promote endothelialization, we investigated the impact of gratings on the biomechanical properties and the replicative capacity of senescent ECs. After cultivation on gridded surfaces, the cells showed significant improvements in terms of adherens junction integrity, cell elongation, and orientation of the actin filaments, as well as enhanced yes-associated protein nuclear translocation and cell proliferation. Our data therefore suggest that micro-structured surfaces with anisotropic topographies may improve long-term endothelialization of CVIs.
Keywords
