Somatic embryogenesis of Sandalwood (Santalum album L.)

Abstract

Sandalwood (Santalum album L.) is native species of Indonesia, especially in East Nusa Tenggara, is one of the twenty two species of the genus Santalum in the world. Sandalwood is an important tree because it has high economic value can produce sandal oil these can be used for perfumes, cosmetics, pharmaceuticals, and are often used in religious ceremonies. In vitro particularly somatic embryogenesis has been widely applied in the propagation of sandalwood. The Objective of this research is to obtain regeneration of sandalwood through somatic embryogenesis using leaves explant from various clones. Medium for embryo induction is MS (Murashige and Skoog, 1962) solid medium containing treatment of 2,4-D (2,4-Dichlorophenoxyacetic acid) at various concentrations. To the media 0,15 mg /l kinetin, 40 g/l sucrose, and 2,5 g/l gelrite were added. Culture were incubated in the dark. Medium for Embryo development (maturation) is MS solid medium containing treatment of BAP (Benzyl-amino-purine) at various concentrations. To the media 0,01 mg /l NAA (Napthalene-acetic-acid), 40 g/l sucrose, and 2,5 g/l gelrite were added. Culture were incubated in the light. To study the specifi c structure of sandalwood somatic embryo early detection was conducted using histological analysis. Results of anova showed that the clones, media, and interaction between clones with media did not signifi cantly affect the development of sandalwood callus percentage. Results of anova showed that the clones and BAP concentration signifi cantly effect to the embryo development of sandalwood.

Keywords

• Somatic embryogenesis, Santalum album, Embryogenic callus, maturation