The Vital Dye CDr10b Labels the Zebrafish Mid-Intestine and Lumen
Veronika Sander,
Shantanu Patke,
Jung Y. Lee,
Young-Tae Chang,
Alan J. Davidson
Affiliations
Veronika Sander
Department of Molecular Medicine & Pathology, University of Auckland, Auckland 1142, New Zealand
Shantanu Patke
Department of Molecular Medicine & Pathology, University of Auckland, Auckland 1142, New Zealand
Jung Y. Lee
Department of Chemistry & NUS MedChem Program of Life Sciences Institute, National University of Singapore and Singapore Bioimaging Consortium, A* STAR, Singapore 138667, Singapore
Young-Tae Chang
Department of Chemistry & NUS MedChem Program of Life Sciences Institute, National University of Singapore and Singapore Bioimaging Consortium, A* STAR, Singapore 138667, Singapore
Alan J. Davidson
Department of Molecular Medicine & Pathology, University of Auckland, Auckland 1142, New Zealand
We describe the use of the fluorescent reporter compound CDr10b to label mid-intestinal structures in zebrafish larvae after simple immersion. CDr10b is deposited into the gut where it initially fills the lumen and is excreted. Using laser-mediated injury of the intestine, we show that CDr10b provides a useful readout of the integrity and repair of the epithelial cell barrier. In addition, CDr10b specifically labels the absorptive mid-intestine segment that is analogous to the mammalian small intestine. By perturbing retinoic acid signaling, which regulates the size of the mid-intestine segment, we show that CDr10b is a valuable tool to rapidly assess developmental malformations of the intestine in live animals.
