Common Genotypes of Human Papillomavirus in East Azerbaijan Population using HPV Direct Flow CHIP Kit

Abstract

Introduction: Human Papilloma Virus (HPV) is one of the most common sexually transmitted viruses and the main cause of urogenital tract cancers. Cervical cancer is the fourth common cause of cancer death in women and in more than 90% is associated with a persistent infection with one of the High-Risk HPV types. This study was performed with aim to determine HPV genotypes in DNAs extracted from tissue samples of people suspected to HPV infection. Methods: In this cross-sectional epidemiological study, 201 tissue samples were obtained from people suspected to HPV infection that had referred to Dr. Mozaffari pathobiology laboratory for diagnosis in Tabriz from 2016-2019. Viral DNA was extracted using high pure viral nucleic acid extraction kit and genotyping was studied using HPV Direct Flow CHIP Kit. Data were analyzed by Excel software. Results: Viral DNA amplification was observed in 45 samples (73%) and considered as HPV-positive. Among them, 8 samples (6%) were infected by high-risk types, 36 samples (34%) by both high & low-risk types and 56 samples (60%) by low-risk types. HPV types 6, 11 and 43 with frequency f 107, 23 and 10, respectively out of 145 people were identified as the most common low-risk types and HPV-18, 39 and 16 with frequency of 23, 11 and 10, respectively were identified as the common high-risk types. Most of the infected women were in the age range of 25-35 years and the age range for men was 35-40 years. Conclusion: This study showed that determining the genotypes of HPV is considered as an important step in the management of HPV-infected people, especially people with persistent infection by High-risk types.

Keywords

• direct flow chip kit
• genotype
• human papillomavirus
• prevalence