Ketamine induced gut microbiota dysbiosis and barrier and hippocampal dysfunction in rats
Lei Xie,
Zelin Zhuang,
Baowen Guo,
Yuehua Huang,
Xiaoyan Shi,
Zikai Huang,
Ziquan Xu,
Yanbin Chen,
Yuyin Cao,
Yanmin Zheng,
Renhua Wu,
Shuhua Ma
Affiliations
Lei Xie
Department of Radiology, The First Affiliated Hospital of Shantou University Medical College, Shantou 515041, China; Laboratory of Molecular Imaging & Laboratory of Molecular Cardiology, The First Affiliated Hospital of Shantou University Medical College, Shantou 515041, China
Zelin Zhuang
Department of Radiology, The First Affiliated Hospital of Shantou University Medical College, Shantou 515041, China; Laboratory of Molecular Imaging & Laboratory of Molecular Cardiology, The First Affiliated Hospital of Shantou University Medical College, Shantou 515041, China
Baowen Guo
Department of Radiology, The First Affiliated Hospital of Shantou University Medical College, Shantou 515041, China; Laboratory of Molecular Imaging & Laboratory of Molecular Cardiology, The First Affiliated Hospital of Shantou University Medical College, Shantou 515041, China
Yuehua Huang
Department of Radiology, The First Affiliated Hospital of Shantou University Medical College, Shantou 515041, China; Laboratory of Molecular Imaging & Laboratory of Molecular Cardiology, The First Affiliated Hospital of Shantou University Medical College, Shantou 515041, China
Xiaoyan Shi
Department of Radiology, The First Affiliated Hospital of Shantou University Medical College, Shantou 515041, China
Zikai Huang
Department of Radiology, The First Affiliated Hospital of Shantou University Medical College, Shantou 515041, China; Laboratory of Molecular Imaging & Laboratory of Molecular Cardiology, The First Affiliated Hospital of Shantou University Medical College, Shantou 515041, China
Ziquan Xu
Department of Radiology, The First Affiliated Hospital of Shantou University Medical College, Shantou 515041, China; Laboratory of Molecular Imaging & Laboratory of Molecular Cardiology, The First Affiliated Hospital of Shantou University Medical College, Shantou 515041, China
Yanbin Chen
Department of Radiology, The First Affiliated Hospital of Shantou University Medical College, Shantou 515041, China; Laboratory of Molecular Imaging & Laboratory of Molecular Cardiology, The First Affiliated Hospital of Shantou University Medical College, Shantou 515041, China
Yuyin Cao
Department of Radiology, The First Affiliated Hospital of Shantou University Medical College, Shantou 515041, China; Laboratory of Molecular Imaging & Laboratory of Molecular Cardiology, The First Affiliated Hospital of Shantou University Medical College, Shantou 515041, China
Yanmin Zheng
Department of Radiology, The First Affiliated Hospital of Shantou University Medical College, Shantou 515041, China; Laboratory of Molecular Imaging & Laboratory of Molecular Cardiology, The First Affiliated Hospital of Shantou University Medical College, Shantou 515041, China
Renhua Wu
Department of Radiology, The Second Affiliated Hospital, Medical College of Shantou University, Shantou, China; Corresponding author
Shuhua Ma
Department of Radiology, The First Affiliated Hospital of Shantou University Medical College, Shantou 515041, China; Laboratory of Molecular Imaging & Laboratory of Molecular Cardiology, The First Affiliated Hospital of Shantou University Medical College, Shantou 515041, China; Corresponding author
Summary: The microbiota-gut-brain axis (MGBA) plays a pivotal role in drug addiction. However, the pathophysiological mechanism of MGBA in ketamine addiction remains elusive. The present study investigated the ketamine-induced gut microbiota disorders, intestinal barrier dysfunction, and the alterations in brain function, using a conditioned place preference (CPP) model of ketamine addiction in rats. Compared with the control group, ketamine induced decreased amplitude of low-frequency fluctuation (ALFF) values in the hippocampus, and pyknotic nuclei and concentrated cytoplasm in hippocampal neurons, as well as alterations in gut microbiota composition, shortened ileum villi, and thinner colonic mucosa. We also found that the abundance of gut microbiota exhibited correlations with CPP score, hippocampal ALFF value, length of ileum villi, and thickness of colonic mucosa. Our findings provide evidence for abnormal alterations in the MGBA of ketamine-addicted rats, which improves our understating of the mechanism of ketamine addiction and the potential for developing new therapeutic strategies.
