Ophiopogonin B inhibits proliferation and migration of human esophageal squamous cell carcinoma cell line EC9706

Abstract

Objective To explore the effect of ophiopogonin B(OP-B) on the biological behavior of esophageal squamous cell carcinoma cell line(EC9706) and its mechanism related to the regulation of Down syndrome cell adhesion molecule antisense 1 (DSCAM-AS1) and miR-199a-3p expression. Methods EC9706 cells were divided into control group, experiment groups with low, medium and high dosage of OP-B, si-NC group, si-DSCAM-AS1 group and (pcDNA-DSCAM-AS1+OP-B) group. Colony formation and CCK-8 method were used to detect EC9706 proliferation. Flow cytometry and scratch healing test were used to analyze the apoptosis rate and migration of EC9706 cells. RT-qPCR was performed to detect the expression of DSCAM-AS1 and miR-199a-3p. The targeting relationship between DSCAM-AS1 and miR-199a-3p was verified using dual luciferase reporter gene experiment. Results OP-B reduced EC9706 viability, colony formation counting, migration distance and DSCAM-AS1 expression (P＜0.05), increased cell apoptosis rate and miR-199a-3p expression (P＜0.05). DSCAM-AS1 interacted directly with miR-199a-3p. After interference to DSCAM-AS1 expression, the cell viability, colony formation and migration distance of EC9706 cells were significantly reduced(P＜0.05), while apoptosis rates were significantly increased(P＜0.05). Over-expression of DSCAM-AS1 antagonized the effects of OP-B on the proliferation, apoptosis and migration of EC9706 cells(P＜0.05). Conclusions OP-B inhibits proliferation and migration of esophageal squamous cell carcinoma cells and induces cell apoptosis. The mechanism is potentially explained by down-regulating DSCAM-AS1/miR-199a-3p pathway.

Keywords

• ophiopogonin b|esophageal squamous cell carcinoma|dscam-as1|mir-199a-3p|migration