Ion-Exclusion/Cation-Exchange Chromatography Using Dual-Ion-Exchange Groups for Simultaneous Determination of Inorganic Ionic Nutrients in Fertilizer Solution Samples for the Management of Hydroponic Culture
Daisuke Kozaki,
Yuki Sago,
Taku Fujiwara,
Masanobu Mori,
Chihiro Kubono,
Tougo Koga,
Yuta Mitsui,
Tomotaka Tachibana
Affiliations
Daisuke Kozaki
Department of Chemistry and Biotechnology, Faculty of Science and Technology, Kochi University, 2-5-1 Akebono-cho, Kochi 780-8520, Japan
Yuki Sago
Graduate School of Sciences and Technology for Innovation, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan
Taku Fujiwara
Department of Environmental Engineering, Kyoto University, C1-222 Nishikyo-ku, Kyoto 615-8540, Japan
Masanobu Mori
Department of Chemistry and Biotechnology, Faculty of Science and Technology, Kochi University, 2-5-1 Akebono-cho, Kochi 780-8520, Japan
Chihiro Kubono
Department of Chemistry and Biotechnology, Faculty of Science and Technology, Kochi University, 2-5-1 Akebono-cho, Kochi 780-8520, Japan
Tougo Koga
Department of Chemistry and Biotechnology, Faculty of Science and Technology, Kochi University, 2-5-1 Akebono-cho, Kochi 780-8520, Japan
Yuta Mitsui
Department of Chemistry and Biotechnology, Faculty of Science and Technology, Kochi University, 2-5-1 Akebono-cho, Kochi 780-8520, Japan
Tomotaka Tachibana
Department of Chemistry and Biotechnology, Faculty of Science and Technology, Kochi University, 2-5-1 Akebono-cho, Kochi 780-8520, Japan
In this study, ion-exclusion/cation-exchange chromatography (IEC/CEC) using dual-ion-exchange groups (carboxy and sulfo groups) for the simultaneous determination of anions (SO42−, Cl−, NO3−, and HPO42−) and cations (Na+, NH4+, K+, Mg2+, and Ca2+) was developed. By using the combination of dual-ion-exchange groups, simultaneous separation of inorganic ions with HPO42− was achieved that was impossible by the conventional IEC/CEC based on the single-ion-exchange group (carboxy group). This method was applied to the monitoring of inorganic ionic nutrients in fertilizer solution samples in hydroponic culture. As a result, a higher peak resolution of inorganic anions and cations with phosphate ion using IEC/CEC with dual-ion-exchange groups was achieved in the absence of matrix effects. In addition, the developed method helps to understand the behavior of ionic nutrients in fertilizer solution during hydroponic cultivation and is potentially useful for the individual fertilization of ionic nutrients.
