Nature Communications (Apr 2020)

A live cell reporter of exosome secretion and uptake reveals pathfinding behavior of migrating cells

  • Bong Hwan Sung,
  • Ariana von Lersner,
  • Jorge Guerrero,
  • Evan S. Krystofiak,
  • David Inman,
  • Roxanne Pelletier,
  • Andries Zijlstra,
  • Suzanne M. Ponik,
  • Alissa M. Weaver

DOI
https://doi.org/10.1038/s41467-020-15747-2
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 15

Abstract

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A prior live-cell exosome reporter showed dim fluorescence and could not be expressed stably, limiting its usefulness. Here the authors stabilise the reporter to allow long-term tracking of exosomes, and incorporate a second fluorophore to visualise the entire exosome lifecycle.