PLASMID PROFILES OF BACILLUS GENUS ANTAGONISTIC STRAINS

Abstract

The aim of the study was to investigate plasmid profiles and to compare the results of the Gram-positive Bacillus genus bacteria plasmid isolation efficiency by various methods. Methods. Strains of B. thuringiensis and B. subtilis were used. Plasmids isolation from the cells was carried out using alkaline Kado and Liu method, Jensen method and Cross method. Results. Plasmid profiles study of B. thuringiensis and B. subtilis strains revealed that 90% of strains carried from 5 to 7 extrachromosomal genetic elements of different sizes. Conclusion. Isolated plasmids can be divided into two groups: small plasmids, about 10 kb in size and megaplasmids 100 to 200 kb. The most suitable method for the study and complete picture of B. thuringiensis or B. subtilis plasmid profiles was Jensen method adapted for bacillus megaplasmids isolation.

Keywords

• bacillus thuringiensis
• bacillus subtilis
• плазміди
• плазмідні профілі