Structural and functional analysis of an inter-Spike bivalent neutralizing antibody against SARS-CoV-2 variants

Yaning Li; Qing Fan; Bing Zhou; Yaping Shen; Yuanyuan Zhang; Lin Cheng; Furong Qi; Shuo Song; Yingying Guo; Renhong Yan; Bin Ju; Zheng Zhang

iScience (Jun 2022)

Structural and functional analysis of an inter-Spike bivalent neutralizing antibody against SARS-CoV-2 variants

Yaning Li,
Qing Fan,
Bing Zhou,
Yaping Shen,
Yuanyuan Zhang,
Lin Cheng,
Furong Qi,
Shuo Song,
Yingying Guo,
Renhong Yan,
Bin Ju,
Zheng Zhang

Affiliations

Yaning Li: Center for Infectious Disease Research, Westlake Laboratory of Life Sciences and Biomedicine, Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, Zhejiang Province, Hangzhou 310024, China; Beijing Advanced Innovation Center for Structural Biology, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China
Qing Fan: Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, Guangdong Province, Shenzhen 518112, China; The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Guangdong Province, Shenzhen 518112, China
Bing Zhou: Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, Guangdong Province, Shenzhen 518112, China; The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Guangdong Province, Shenzhen 518112, China
Yaping Shen: Center for Infectious Disease Research, Westlake Laboratory of Life Sciences and Biomedicine, Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, Zhejiang Province, Hangzhou 310024, China
Yuanyuan Zhang: Center for Infectious Disease Research, Westlake Laboratory of Life Sciences and Biomedicine, Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, Zhejiang Province, Hangzhou 310024, China
Lin Cheng: Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, Guangdong Province, Shenzhen 518112, China; The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Guangdong Province, Shenzhen 518112, China
Furong Qi: Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, Guangdong Province, Shenzhen 518112, China; The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Guangdong Province, Shenzhen 518112, China
Shuo Song: Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, Guangdong Province, Shenzhen 518112, China; The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Guangdong Province, Shenzhen 518112, China
Yingying Guo: Center for Infectious Disease Research, Westlake Laboratory of Life Sciences and Biomedicine, Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, Zhejiang Province, Hangzhou 310024, China
Renhong Yan: Department of Biochemistry, School of Medicine, Southern University of Science and Technology, Guangdong Province, Shenzhen 518055, China; Corresponding author
Bin Ju: Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, Guangdong Province, Shenzhen 518112, China; The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Guangdong Province, Shenzhen 518112, China; Guangdong Key Laboratory for Anti-infection Drug Quality Evaluation, Guangdong Province, Shenzhen 518112, China; Corresponding author
Zheng Zhang: Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, Guangdong Province, Shenzhen 518112, China; The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Guangdong Province, Shenzhen 518112, China; Guangdong Key Laboratory for Anti-infection Drug Quality Evaluation, Guangdong Province, Shenzhen 518112, China; Shenzhen Research Center for Communicable Disease Diagnosis and Treatment of Chinese Academy of Medical Science, Guangdong Province, Shenzhen 518112, China; Corresponding author

Journal volume & issue: Vol. 25, no. 6
p. 104431

Abstract

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Summary: The different variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have attracted most public concern because they caused “wave and wave” COVID-19 pandemic. The initial step of viral infection is mediated by the SARS-CoV-2 Spike (S) protein, which mediates the receptor recognition and membrane fusion between virus and host cells. Neutralizing antibodies (nAbs) targeting the S protein of SARS-CoV-2 have become promising candidates for clinical intervention strategy, while multiple studies have shown that different variants have enhanced infectivity and antibody resistance. Here, we explore the structure and function of STS165, a broadly inter-Spike bivalent nAb against SARS-CoV-2 variants and even SARS-CoV, contributing to further understanding of the working mechanism of nAbs.

Published in iScience

ISSN: 2589-0042 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science
Website: http://www.cell.com/iscience/home

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