Elimination of the yellow pigment gene PSY-E2 tightly linked to the Fusarium head blight resistance gene Fhb7 from Thinopyrum ponticum
Xuefeng Li,
Dong Li,
Yu Xuan,
Ziming He,
Lanfei Zhao,
Yongchao Hao,
Wenyang Ge,
Shoushen Xu,
Bingqian Hou,
Biao Wang,
Jun Guo,
Wenwen Liu,
Mingzhu Li,
Yi Han,
Cunyao Bo,
Yinguang Bao,
Zengjun Qi,
Steven S. Xu,
Guihua Bai,
Hongwei Wang,
Lingrang Kong
Affiliations
Xuefeng Li
State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, Shandong, China
Dong Li
State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, Shandong, China
Yu Xuan
State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, Shandong, China
Ziming He
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China
Lanfei Zhao
Department of Agronomy, Kansas State University, 2004 Throckmorton Hall, Manhattan, KS 66506, USA
Yongchao Hao
State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, Shandong, China
Wenyang Ge
State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, Shandong, China
Shoushen Xu
State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, Shandong, China
Bingqian Hou
State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, Shandong, China
Biao Wang
State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, Shandong, China
Jun Guo
Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong, China
Wenwen Liu
State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, Shandong, China
Mingzhu Li
State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, Shandong, China
Yi Han
State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, Shandong, China
Cunyao Bo
State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, Shandong, China
Yinguang Bao
State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, Shandong, China
Zengjun Qi
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China
Steven S. Xu
Cereal Crops Research Unit, Red River Valley Agricultural Research Center, USDA-ARS, Fargo, ND 58102, USA
Guihua Bai
USDA, Hard Winter Wheat Genetics Research Unit, 4008 Throckmorton Hall, Manhattan, KS 66506, USA; Department of Agronomy, Kansas State University, 2004 Throckmorton Hall, Manhattan, KS 66506, USA
Hongwei Wang
State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, Shandong, China; Corresponding authors.
Lingrang Kong
State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, Shandong, China; Corresponding authors.
Fhb7 is a major gene that was transferred from Thinopyrum ponticum to chromosome 7D of wheat (Triticum aestivum) and confers resistance to both Fusarium head blight (FHB) and Fusarium crown rot (FCR). However, Fhb7 is tightly linked to the PSY-E2 gene, which causes yellow flour, limiting its application in breeding. To break this linkage, marker K-PSY was developed for tagging PSY-E2 and used with Fhb7 markers to identify recombination between the two genes. Screening 21,000 BC1F2 backcross progeny (Chinese Spring ph1bph1b*2/SDAU 2028) revealed two Fhb7+ wheat-Tp7el2L lines, Shannong 2–16 and Shannong 16–1, that carry a desired truncated Fhb7+ translocation segment without PSY-E2. The two lines show levels of resistance to FHB and FCR similar to those of the original translocation line SDAU 2028, but have white flour. To facilitate Fhb7 use in wheat breeding, STS markers were developed and used to isolate Fhb7 on a truncated Tp7el2 translocation segment. Near-isogenic lines carrying the Fhb7+ segment were generated in the backgrounds of three commercial cultivars, and Fhb7+ lines showed increased FHB and FCR resistance without yield penalty. The breakage of the tight linkage between Fhb7 and PSY-E2 via homoeologous recombination provides genetic resources for improvement of wheat resistance to FHB and FCR and permit the large-scale deployment of Fhb7 in breeding using marker-assisted selection.
