Brazilian Journal of Biology ()

Effect of histological processing and methacrylate sectioning on the area of gill tissue in teleost

  • AL. Cruz,
  • MN. Fernandes,
  • SF. Perry

DOI
https://doi.org/10.1590/s1519-69842009000200022
Journal volume & issue
Vol. 69, no. 2
pp. 385 – 387

Abstract

Read online

Deformation of biological tissues may occur during histological processing and results in loss of accuracy when quantitative information about cells, tissues and organs is necessary. In this study, the gill tissue from armored catfish (Pterygoplichthys anisitsi) was quantified in each step of processing using the stereological principles. During processing for glycol methacrylate embedding, gill tissue from shrinks significantly but regains its original dimensions after sectioning.

Keywords