Journal of Pain Research (Nov 2020)
Decreased MiR-485-5p Contributes to Inflammatory Pain Through Post-Transcriptional Upregulation of ASIC1 in Rat Dorsal Root Ganglion
Abstract
Meijie Xu,1 Rui Wu,1,2 Ling Zhang,1 Hong-Yan Zhu,1 Guang-Yin Xu,1,2 Wenxia Qian,1 Ping-An Zhang1,2 1Department of Respiratory and Critical Care Medicine, Affiliated Zhangjiagang Hospital of Soochow University, Zhangjiagang 215600, People’s Republic of China; 2Center for Translational Pain Medicine, Institute of Neuroscience, Soochow University, Suzhou 215123, People’s Republic of ChinaCorrespondence: Ping-An ZhangCenter for Translational Pain Medicine, Institute of Neuroscience, Soochow University, Suzhou 215123, People’s Republic of ChinaTel +86-15006281769Email [email protected] QianDepartment Of Respiratory and Critical Care Medicine, Affiliated Zhangjiagang Hospital of Soochow University, Zhangjiagang 215600, People’s Republic of ChinaTel +86-13952440813Email [email protected]: Inflammatory pain is the most common type of pain treated clinically. However, the currently available treatments for inflammatory pain have limited effects and can cause severe side effects. The aim of this study is to describe the effect of miRNA-485-5p on osteoarthritis-related inflammatory pain.Methods: Paw withdrawal threshold (PWT) of rats was measured by von Frey filaments. The expressions of miRNA-485-5p and acid-sensing ion channel 1 (ASIC1) in the dorsal root ganglion (DRG) were measured with real-time quantitative PCR and Western blotting analysis. Fluorescent in situ hybridization and fluorescent immunohistochemistry were employed to detect expression of miRNA-485-5p, acid-sensing ion channelASIC1 and co-location of miRNA-485-5p with ASIC1.Results: The PWT of rats was significantly reduced after complete Freund’s adjuvant (CFA) injection. The miRNA-485-5p expression level clearly decreased while the ASIC1 expression level was upregulated in the L4-6 dorsal root ganglion (DRG) of CFA rats. MiRNA-485-5p and ASIC1 were co-expressed in the same DRG cells of CFA rats. Amiloride, an inhibitor of ASIC1, clearly increased the PWT of CFA rats. Further, miRNA-485-5p agomir reversed the upregulation of ASICI1 and alleviated CFA-induced mechanical hypersensitivity of CFA rats.Conclusion: These results suggest that reduced expression of miRNA-485-5p contributes to inflammatory pain through upregulating ASIC1 expression, implying a promising strategy for pain therapy.Keywords: miR-485-5p, ASIC1, dorsal root ganglion (DRG), inflammatory pain
