mdm-miR828 Participates in the Feedback Loop to Regulate Anthocyanin Accumulation in Apple Peel

Bo Zhang; Bo Zhang; Hui-Juan Yang; Hui-Juan Yang; Ya-Zhou Yang; Ya-Zhou Yang; Zhen-Zhen Zhu; Zhen-Zhen Zhu; Ya-Nan Li; Ya-Nan Li; Dong Qu; Zheng-Yang Zhao; Zheng-Yang Zhao

doi:10.3389/fpls.2020.608109

Frontiers in Plant Science (Dec 2020)

mdm-miR828 Participates in the Feedback Loop to Regulate Anthocyanin Accumulation in Apple Peel

Bo Zhang,
Bo Zhang,
Hui-Juan Yang,
Hui-Juan Yang,
Ya-Zhou Yang,
Ya-Zhou Yang,
Zhen-Zhen Zhu,
Zhen-Zhen Zhu,
Ya-Nan Li,
Ya-Nan Li,
Dong Qu,
Zheng-Yang Zhao,
Zheng-Yang Zhao

Affiliations

Bo Zhang: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, China
Bo Zhang: Apple Engineering and Technology Research Center of Shaanxi Province, Northwest A&F University, Yangling, China
Hui-Juan Yang: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, China
Hui-Juan Yang: Apple Engineering and Technology Research Center of Shaanxi Province, Northwest A&F University, Yangling, China
Ya-Zhou Yang: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, China
Ya-Zhou Yang: Apple Engineering and Technology Research Center of Shaanxi Province, Northwest A&F University, Yangling, China
Zhen-Zhen Zhu: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, China
Zhen-Zhen Zhu: Apple Engineering and Technology Research Center of Shaanxi Province, Northwest A&F University, Yangling, China
Ya-Nan Li: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, China
Ya-Nan Li: Apple Engineering and Technology Research Center of Shaanxi Province, Northwest A&F University, Yangling, China
Dong Qu: Shaanxi Key Laboratory Bio-resources, College of Bioscience and Engineering, Shaanxi University of Technology, Hanzhong, China
Zheng-Yang Zhao: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, China
Zheng-Yang Zhao: Apple Engineering and Technology Research Center of Shaanxi Province, Northwest A&F University, Yangling, China

DOI: https://doi.org/10.3389/fpls.2020.608109
Journal volume & issue: Vol. 11

Abstract

Read online

Anthocyanins are responsible for the red pigmentation in the peel of apple (Malus × domestica Borkh.) fruit. Relatively few studies have investigated anthocyanins at the posttranscriptional level. MicroRNAs play an important role in plant growth and development by regulating gene expression at the posttranscriptional level. In this study, mdm-miR828 showed a relatively low expression level during the rapid fruit coloration period. However, the mdm-miR828 expression level increased in the late fruit coloration stage. Overexpression of mdm-miR828 inhibited anthocyanin synthesis in apple and Arabidopsis. Dual-luciferase and yeast one-hybrid assays showed that MdMYB1 is capable of binding to the promoter of mdm-MIR828b to promote its expression. The results indicate that mdm-miR828 is involved in a feedback regulatory mechanism associated with anthocyanin accumulation in apple. In addition, mdm-miR828 is involved in the inhibition of anthocyanin accumulation in response to high temperature.

Published in Frontiers in Plant Science

ISSN: 1664-462X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Agriculture: Plant culture
Website: https://www.frontiersin.org/journals/plant-science

About the journal

Abstract

Keywords