Proteomic dataset of the organohalide-respiring bacterium Dehalococcoides mccartyi strain CBDB1 grown on hexachlorobenzene as electron acceptor
Christian L. Schiffmann,
Wolfgang Otto,
Rasmus Hansen,
Per Halkjær Nielsen,
Lorenz Adrian,
Jana Seifert,
Martin von Bergen,
Nico Jehmlich
Affiliations
Christian L. Schiffmann
Department of Molecular Systems Biology, Helmholtz Centre for Environmental Research–UFZ, Permoserstr. 15, 04318 Leipzig, Germany
Wolfgang Otto
Department of Molecular Systems Biology, Helmholtz Centre for Environmental Research–UFZ, Permoserstr. 15, 04318 Leipzig, Germany
Rasmus Hansen
Aalborg University, Department of Biotechnology, Chemistry and Environmental Engineering, Sohngårdsholmsvej 49, 9000 Aalborg, Denmark
Per Halkjær Nielsen
Aalborg University, Department of Biotechnology, Chemistry and Environmental Engineering, Sohngårdsholmsvej 49, 9000 Aalborg, Denmark
Lorenz Adrian
Department Isotope Biogeochemistry, Helmholtz Centre for Environmental Research–UFZ, Permoserstr. 15, 04318 Leipzig, Germany
Jana Seifert
Institute of Animal Science, Hohenheim University, 70593 Stuttgart, Germany
Martin von Bergen
Department of Molecular Systems Biology, Helmholtz Centre for Environmental Research–UFZ, Permoserstr. 15, 04318 Leipzig, Germany; Aalborg University, Department of Biotechnology, Chemistry and Environmental Engineering, Sohngårdsholmsvej 49, 9000 Aalborg, Denmark
Nico Jehmlich
Department of Molecular Systems Biology, Helmholtz Centre for Environmental Research–UFZ, Permoserstr. 15, 04318 Leipzig, Germany; Corresponding author. Tel.: +49 341 235 4767; fax: +49 341 235 450823.
The proteome of the anaerobic organohalide-respiring bacterium Dehalococcoides mccartyi strain CBDB1 was analyzed by nano liquid chromatography coupled to mass spectrometry (LC-MS/MS). Two different preparation methods, (i) in-solution and (ii) in-gel proteolytic digestion were assessed to elucidate the core and the functional proteome of bacterial cultures grown in synthetic anaerobic medium with hexachlorobenzene as sole electron acceptor. A detailed analysis of the data presented is available (Schiffmann et al., 2014) [1]. Keywords: Proteomic reference profile, Dehalococcoides mccartyi strain, CBDB1, Shotgun proteomics, Spectral library
