Screening microbially produced Δ9-tetrahydrocannabinol using a yeast biosensor workflow

William M. Shaw; Yunfeng Zhang; Xinyu Lu; Ahmad S. Khalil; Graham Ladds; Xiaozhou Luo; Tom Ellis

doi:10.1038/s41467-022-33207-x

Nature Communications (Sep 2022)

Screening microbially produced Δ9-tetrahydrocannabinol using a yeast biosensor workflow

William M. Shaw,
Yunfeng Zhang,
Xinyu Lu,
Ahmad S. Khalil,
Graham Ladds,
Xiaozhou Luo,
Tom Ellis

Affiliations

William M. Shaw: Biological Design Center, Boston University
Yunfeng Zhang: Center for Synthetic Biochemistry, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences
Xinyu Lu: Department of Bioengineering, Imperial College London
Ahmad S. Khalil: Biological Design Center, Boston University
Graham Ladds: Department of Pharmacology, University of Cambridge
Xiaozhou Luo: Center for Synthetic Biochemistry, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences
Tom Ellis: Department of Bioengineering, Imperial College London

DOI: https://doi.org/10.1038/s41467-022-33207-x
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 10

Abstract

Read online

Microbial production of cannabinoids promises a cheaper and more sustainable route to these important therapeutic molecules, but strain improvement and screening is challenging. Here, the authors develop a yeast-based Δ9-tetrahydrocannabinol (THC) biosensor for screening microbial mutant libraries.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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