Cloning, Expression and Characterization of an Esterase Gene in a Metagenomic Library of Traditional Fermented Food

Abstract

Esterase is an industrial enzyme that is widely used in food, medicine, fine chemicals. The total genomic DNA was extracted from traditional fermented food in China to construct a metagenomic library that included a novel esterase gene (est_115). Sequence homology analysis showed that the highest homology with the carboxylester hydrolase from Pseudomonas lutea was 38%, indicating that esterase belongs to a new class of esterases. Then, an est_115 gene recombinant expression vector was constructed and expressed. The Est_115 had higher catalytic activity to p-nitrophenol ester, with a short acyl-carbon chain. The enzyme can maintain high catalytic activity and salt tolerance in 10%–18% NaCl, suggesting that this novel esterase can be used in processing food using high osmotic pressure.

Keywords

• traditional food
• metagenomics
• esterase
• cloning
• properties