CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum

Yu Jiang; Fenghui Qian; Junjie Yang; Yingmiao Liu; Feng Dong; Chongmao Xu; Bingbing Sun; Biao Chen; Xiaoshu Xu; Yan Li; Renxiao Wang; Sheng Yang

doi:10.1038/ncomms15179

Nature Communications (May 2017)

CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum

Yu Jiang,
Fenghui Qian,
Junjie Yang,
Yingmiao Liu,
Feng Dong,
Chongmao Xu,
Bingbing Sun,
Biao Chen,
Xiaoshu Xu,
Yan Li,
Renxiao Wang,
Sheng Yang

Affiliations

Yu Jiang: Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
Fenghui Qian: Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
Junjie Yang: Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
Yingmiao Liu: Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
Feng Dong: Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
Chongmao Xu: Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
Bingbing Sun: Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
Biao Chen: Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
Xiaoshu Xu: Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
Yan Li: State Key Laboratory of Bioorganic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences
Renxiao Wang: State Key Laboratory of Bioorganic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences
Sheng Yang: Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

DOI: https://doi.org/10.1038/ncomms15179
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 11

Abstract

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Corynebacterium glutamicum is an important industrial microbe, however it has proven difficult to genetically engineer using Cas9 from Streptococcus pyogenes. Here the authors report effective genome engineering of the bacterium using Cpf1 from Francisella novicida.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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