OncoTargets and Therapy (Feb 2020)
Long Non-Coding RNA HULC Promotes Cervical Cancer Cell Proliferation, Migration and Invasion via miR-218/TPD52 Axis
Abstract
Wenjun Lu,1 Xiaobin Wan,2 Limin Tao,1 Junhui Wan1 1Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi, People’s Republic of China; 2Department of General Surgery, The Third Hospital of Nanchang University, Nanchang 330006, Jiangxi, People’s Republic of ChinaCorrespondence: Limin Tao; Junhui WanDepartment of Obstetrics and Gynecology, The First Affiliated Hospital of Nanchang University, No. 17 Yongwai Zhengjie, Donghu District, Nanchang 330006, Jiangxi, People’s Republic of ChinaTel +86-13879136013; +86-13970974546Email [email protected]; [email protected]: Long non-coding RNAs (lncRNAs) have been identified as important players in tumorigenesis. LncRNA highly upregulated in liver cancer (HULC) has been identified as a key regulator in the progression of various cancers. However, the functional role and the mechanisms of HULC in regulating cervical cancer cell behavior remain unclear.Methods: HULC expression, miR-218 expression and TPD52 mRNA level in cervical cancer cells were examined by qRT-PCR. Cell proliferation was evaluated by MTT assay. Cell migration and invasion were examined by Transwell assay. TPD52 protein level was measured by Western blot. Dual-luciferase reporter assay was measured to verify the combination of HULC and miR-218 as well as miR-218 and TPD52.Results: HULC expression was upregulated in cervical cancer cell lines, and HULC promoted cervical cancer cell proliferation, migration and invasion. Mechanistically, HULC acted as a sponge of miR-218 to elevate expression of TPD52, a target of miR-218, and thereby promoted cervical cancer cell proliferation, migration, and invasion.Conclusion: HULC promotes cervical cancer cell proliferation, migration and invasion via miR-218/TPD52 axis.Keywords: HULC, miR-218, TPD52, cervical cancer cell proliferation
