Innovative Animal Model of DSS-Induced Ulcerative Colitis in Pseudo Germ-Free Mice
Sona Gancarcikova,
Stanislav Lauko,
Gabriela Hrckova,
Zuzana Andrejcakova,
Vanda Hajduckova,
Marian Madar,
Livia Kolesar Fecskeova,
Dagmar Mudronova,
Kristina Mravcova,
Gabriela Strkolcova,
Radomira Nemcova,
Jana Kacirova,
Andrea Staskova,
Stefan Vilcek,
Alojz Bomba
Affiliations
Sona Gancarcikova
Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Kosice, 041 81 Kosice, Slovakia
Stanislav Lauko
Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Kosice, 041 81 Kosice, Slovakia
Gabriela Hrckova
Institute of Parasitology, Slovak Academy of Sciences, 041 81 Kosice, Slovakia
Zuzana Andrejcakova
Department of Biology and Physiology, University of Veterinary Medicine and Pharmacy in Kosice, 041 81 Kosice, Slovakia
Vanda Hajduckova
Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Kosice, 041 81 Kosice, Slovakia
Marian Madar
Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Kosice, 041 81 Kosice, Slovakia
Livia Kolesar Fecskeova
Institute of Microbiology, Czech Academy of Sciences, Centre Algatech, 379 01 Trebon, Czech Republic
Dagmar Mudronova
Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Kosice, 041 81 Kosice, Slovakia
Kristina Mravcova
Department of Epizootology, Parasitology and Protection of One Health, University of Veterinary Medicine and Pharmacy in Kosice, 041 81 Kosice, Slovakia
Gabriela Strkolcova
Department of Epizootology, Parasitology and Protection of One Health, University of Veterinary Medicine and Pharmacy in Kosice, 041 81 Kosice, Slovakia
Radomira Nemcova
Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Kosice, 041 81 Kosice, Slovakia
Jana Kacirova
Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Kosice, 041 81 Kosice, Slovakia
Andrea Staskova
Department of Stomatology and Maxilofacial Surgery, Pavol Jozef Safarik University in Kosice, 041 81 Kosice, Slovakia
Stefan Vilcek
Department of Epizootology, Parasitology and Protection of One Health, University of Veterinary Medicine and Pharmacy in Kosice, 041 81 Kosice, Slovakia
Alojz Bomba
Institute of Experimental Medicine, Faculty of Medicine, Pavol Jozef Safarik University in Kosice, 041 81 Kosice, Slovakia
The aim of this study was to investigate the use of a standardized animal model subjected to antibiotic treatment, and the effects of this treatment on the course of dextran sodium sulphate (DSS)-induced colitis in mice. By decontamination with selective antibiotics and observation of pathogenesis of ulcerative colitis (UC) induced chemically by exposure of mice to various concentrations of DSS, we obtained an optimum animal PGF model of acute UC manifested by mucin depletion, epithelial degeneration and necrosis, leading to the disappearance of epithelial cells, infiltration of lamina propria and submucosa with neutrophils, cryptitis, and accompanied by decreased viability of intestinal microbiota, loss of body weight, dehydration, moderate rectal bleeding, and a decrease in the selected markers of cellular proliferation and apoptosis. The obtained PGF model did not exhibit changes that could contribute to inflammation by means of alteration of the metabolic status and the induced dysbiosis did not serve as a bearer of pathogenic microorganisms participating in development of ulcerative colitis. The inflammatory process was induced particularly by exposure to DSS and its toxic action on compactness and integrity of mucosal barrier in the large intestine. This offers new possibilities of the use of this animal model in studies with or without participation of pathogenic microbiota in IBD pathogenesis.