Genome-Wide Identification of Strawberry Metal Tolerance Proteins and Their Expression under Cadmium Toxicity
Muhammad Salman Haider,
Taha Majid Mahmood Sheikh,
Songtao Jiu,
Muqaddas Aleem,
Waqar Shafqat,
Komal Shoukat,
Nadeem Khan,
Muhammad Jafar Jaskani,
Summar A. Naqvi,
Sezai Ercisli,
Amine Assouguem,
Mohammed Kara,
Riaz Ullah,
Maha Aljabri,
Sameer H. Qari
Affiliations
Muhammad Salman Haider
Department of Horticulture, Ghazi University, Dera Ghazi Khan 32200, Pakistan
Taha Majid Mahmood Sheikh
Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Songtao Jiu
Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
Muqaddas Aleem
Center for Advanced Studies in Agriculture and Food Security, University of Agriculture, Faisalabad 38040, Pakistan
Waqar Shafqat
Department of Forestry, College of Forest Resources, Mississippi State University, Starkville, MI 39759, USA
Komal Shoukat
Department of Environmental Sciences, Government College Women University, Sailkot 51310, Pakistan
Nadeem Khan
Department of Biology, University of Ottawa, 30 Marie Curie, Ottawa, ON K1N 6N5, Canada
Muhammad Jafar Jaskani
Institute of Horticultural Sciences, University of Agriculture, Faisalabad 38040, Pakistan
Summar A. Naqvi
Institute of Horticultural Sciences, University of Agriculture, Faisalabad 38040, Pakistan
Sezai Ercisli
Department of Horticulture, Faculty of Agriculture, Ataturk University, 25240 Erzurum, Turkey
Amine Assouguem
Laboratory of Functional Ecology and Environment, Faculty of Sciences and Technology, Sidi Mohamed Ben Abdellah University, Imouzzer Street, Fez 30000, Morocco
Mohammed Kara
Laboratory of Biotechnology and Conservation and Valorization of Natural Resources (LBCVRN) (ex LBPRN), Sidi Mohamed Ben Abdellah University, Fez 30000, Morocco
Riaz Ullah
Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
Maha Aljabri
Department of Biology, Research Laboratories Centre, Faculty of Applied Science, Umm Al-Qura University, Makkah 24382, Saudi Arabia
Sameer H. Qari
Department of Biology, Al-Jumum University College, Umm Al-Qura University, Makkah 24382, Saudi Arabia
Metal tolerance proteins (MTPs) are divalent cation transporters, known to upkeep the mineral nutrition of plants and heavy metal transport at cell, tissue, or whole plant levels. However, information related to evolutionary relationships and biological functions of MTP genes in strawberry (Fragaria vesca L.) remain elusive. Herein, we identified 12 MTP genes from the strawberry genome and divided them into three main groups (i.e., Zn-MTP, Fe/Zn MTP, and Mn-MTP), which is similar to MTP grouping in Arabidopsis and rice. The strawberry MTPs (FvMTPs) are predicted to be localized in the vacuole, while open reading frame (ORF) length ranged from 1113 to 2589 bp with 370 to 862 amino acids, and possess 4 to 6 transmembrane domains (TMDs), except for FvMTP12 that possessed 16 TMDs. All the FvMTP genes had putative cation efflux and cation diffusion facilitator domains along with a zinc dimerization (ZT-dimer) domain in Mn-MTPs. The collinear analysis suggested their conservation between strawberry and Arabidopsis MTPs. Promoter analysis also demonstrated that some of them might possibly be regulated by hormones and abiotic stress factors. Moreover, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis proposed that FvMTP genes are involved in cation transport and homeostasis. The expression analysis showed that FvMTP1, FvMTP1.1, and FvMTP4 were significantly induced in leaf samples, while FvMTP1.1 and FvMTP4 were significantly regulated in roots of cadmium (Cd)-treated strawberry plants during progressive stress duration. The findings of Cd accumulation depicted that Cd contents were significantly higher in root tissues than that of leaf tissues of strawberry. These results are indicative of their response during the specific duration in Cd detoxification, while further functional studies can accurately verify their specific role.
