Frontiers in Microbiology (Dec 2024)

Improvement of silage characteristics of Lactobacillus salivarius HMC4 and improvement of silage quality of king grass

  • Jinsong Yang,
  • Songsong Zhao,
  • Wenbo Zhi,
  • Tianyu Lu,
  • Huahua Qiao,
  • Wei Liu,
  • Ying Dou,
  • Haisheng Tan,
  • Hanlin Zhou,
  • Hanlin Zhou

DOI
https://doi.org/10.3389/fmicb.2024.1468577
Journal volume & issue
Vol. 15

Abstract

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The effect of HMC4 produced by protoplast fusion on silage was studied. The silage formula was composed of heterozygote HMC4 (Group C), parent Lactobacillus (Group A) and a combination of two parents (Group B). The fermentation quality and microbial composition of each batch of silage were evaluated. The results showed that the propionic acid concentration in group C was the lowest, but the organic acid content in feed was significantly increased. Groups B and C had slightly lower crude fiber levels than group A, while groups A and C had higher levels of soluble sugars. The dynamic observation of C group showed that the nutrient composition of feed changed gradually with the extension of silage period. High-throughput sequencing revealed fluctuations in microbial composition before and after silage. Over time, Lactobacillus became the dominant strain and its numbers steadily increased.

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