In-process monitoring of a tissue-engineered oral mucosa fabricated on a micropatterned collagen scaffold: use of optical coherence tomography for quality control
O. Suebsamarn,
Y. Kamimura,
A. Suzuki,
Y. Kodama,
R. Mizuno,
Y. Osawa,
T. Komatsu,
T. Sato,
K. Haga,
R. Kobayashi,
E. Naito,
M. Kida,
K. Kishimoto,
J. Mizuno,
H. Hayasaki,
K. Izumi
Affiliations
O. Suebsamarn
Division of Biomimetics, Faculty of Dentistry & Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan; Division of Paediatric Dentistry, Faculty of Dentistry & Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan; Children’s Oral Health Department, Institute of Dentistry, Suranaree University of Technology, Nakhonratchasima, Thailand
Y. Kamimura
SCREEN Holdings Co., Ltd., Kyoto, Japan
A. Suzuki
Division of Biomimetics, Faculty of Dentistry & Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan
Y. Kodama
Taki Chemical Co., Ltd., Kakogawa, Hyogo, Japan
R. Mizuno
Komatsuseiki Kosakusho Co., Ltd., Suwa, Nagano, Japan
Y. Osawa
Komatsuseiki Kosakusho Co., Ltd., Suwa, Nagano, Japan
T. Komatsu
Nanograins Co., Ltd., Suwa, Nagano, Japan
T. Sato
Center for Transdisciplinary Research, Institute for Research Promotion, Niigata University, Niigata, Japan
K. Haga
Division of Biomimetics, Faculty of Dentistry & Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan; Division of Reconstructive Surgery for Oral and Maxillofacial Region, Faculty of Dentistry & Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan
R. Kobayashi
Division of Biomimetics, Faculty of Dentistry & Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan
E. Naito
Division of Biomimetics, Faculty of Dentistry & Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan
M. Kida
SCREEN Holdings Co., Ltd., Kyoto, Japan
K. Kishimoto
Department of Nanoscience and Nanoengineering, Graduate School of Advanced Science and Engineering, Waseda University, Tokyo, Japan
J. Mizuno
Research Organisation for Nano and Life Innovation, Waseda University, Tokyo, Japan
H. Hayasaki
Division of Paediatric Dentistry, Faculty of Dentistry & Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan
K. Izumi
Division of Biomimetics, Faculty of Dentistry & Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan; Corresponding author.
Background: We previously reported a novel technique for fabricating dermo-epidermal junction (DEJ)-like micropatterned collagen scaffolds to manufacture an ex vivo produced oral mucosa equivalent (EVPOME) for clinical translation; however, more biomimetic micropatterns are required to promote oral keratinocyte-based tissue engineering/regenerative medicine. In addition, in-process monitoring for quality control of tissue-engineered products is key to successful clinical outcomes. However, evaluating three-dimensional tissue-engineered constructs such as EVPOME is challenging. This study aimed to update our technique to fabricate a more biomimetic DEJ structure of oral mucosa and to investigate the efficacy of optical coherence tomography (OCT) in combination with deep learning for non-invasive EVPOME monitoring. Methods: A picosecond laser-textured microstructure mimicking DEJ on stainless steel was used as a negative mould to fabricate the micropatterned collagen scaffold. During EVPOME manufacturing, OCT was applied twice to monitor the EVPOME and evaluate its epithelial thickness. Findings: Our moulding system resulted in successful micropattern replication on the curved collagen scaffold. OCT imaging visualised the epithelial layer and the underlying micropatterned scaffold in EVPOME, enabling to non-invasively detect specific defects not found before the histological examination. Additionally, a gradual increase in epithelial thickness was observed over time. Conclusion: These findings demonstrate the feasibility of using a stainless-steel negative mould to create a more biomimetic micropattern on collagen scaffolds and the potential of OCT imaging for quality control in oral keratinocyte-based tissue engineering/regenerative medicine.
