大豆卵磷脂中二亚油酰磷脂酰胆碱的分离纯化Separation and purification of dilinoleoylphosphatidylcholine from soybean lecithin

Abstract

以大豆粉末磷脂为原料，采用95%乙醇溶液提纯得到卵磷脂，再采用反相制备色谱系统分离纯化得到二亚油酰磷脂酰胆碱（DLPC）。探讨了洗脱剂种类、洗脱流速、上样量对分离纯化DLPC的影响。结果表明：以甲醇溶液作为洗脱剂进行洗脱时，在甲醇体积分数95%、洗脱流速28 mL/min、上样量0.5 g（以40 g C18柱填充材料为基准）时，DLPC纯度可达91.2%，得率为21.5%；以乙醇溶液作为洗脱剂进行洗脱时，在乙醇体积分数80%、洗脱流速28 mL/min、上样量0.75 g（以40 g C18柱填充材料为基准）时， DLPC纯度可达92.6%，得率为20.1%。从DLPC的纯度考虑，以乙醇溶液作为洗脱剂优于甲醇溶液。 Lecithin (PC) was obtained from soybean powder phospholipids by purification with 95% ethanol solution, and then dilinoleoylphosphatidylcholine (DLPC) was separated and purified by reverse phase chromatography preparation system. The effects of eluent type, flow rate of eluent and loading amount on the separation and purification of DLPC were investigated. The results showed that the purity of DLPC was 91.2% and the yield was 21.5% when methanol solution was used as eluent with a volume fraction of 95%, the flow rate of elution was 28 mL/min and the loading amount was 0.5 g (based on 40 g C18 column material). The purity of DLPC was 92.6% and the yield was 20.1% when ethanol solution was used as eluent with a volume fraction of 80%, the flow rate of elution was 28 mL/min, and the loading amount was 0.75 g(based on 40 g C18 column material). In terms of the purity of DLPC, ethanol solution as the eluent is superior to methanol solution.

Keywords

• 大豆粉末磷脂；二亚油酰磷脂酰胆碱；反相柱色谱；纯化
• soybean powder phospholipids；dilinoleoylphosphatidylcholine; reverse phase column chromatography; purification