Association of single nucleotide polymorphisms of the TLR3 gene with Henoch-Schönlein purpura in children

Abstract

Objective To investigate the association of single nucleotide polymorphisms of the TLR3 gene with susceptibility to Henoch-Schönlein purpura (HSP) and Henoch-Schönlein purpura nephritis (HSPN) in children. Methods A total of 174 children with HSP were enrolled as case group, and 162 healthy children who underwent physical examination during the same period of time were enrolled as control group. The children in the case group were divided into HSP group and HSPN group accor-ding to the presence or absence of renal damage during follow-up. Multiplex polymerase chain reaction was used for targeted capture of the rs35311343, rs121434431, rs199768900, rs768091235, and rs1244010954 loci of the TLR3 gene, high-throughput sequencing was performed for the above loci of all samples, and a statistical analysis was performed for the genotype and gene frequency of each locus. Results There were no significant differences in the genotype and allele frequencies of the rs35311343, rs121434431, rs199768900, rs768091235, and rs1244010954 loci of the TLR3 gene between the case group and the control group (P>0.05). For the case group, there were no significant differences in the genotype and allele frequencies of the rs121434431, rs199768900, rs768091235, and rs1244010954 loci of the TLR3 gene between the HSP group and the HSPN group (P>0.05), while there was a significant difference in the genotype and allele frequencies of the rs35311343 locus between the two groups (χ2=9.492,OR=2.662,95%CI=1.342-5.281,P<0.05). Conclusion The CG genotype of the rs35311343 locus of the TLR3 gene is associated with renal involvement in children with HSP, and allele G may be a susceptibility gene for HSPN.

Keywords

• purpura, schoenlein-henoch|toll-like receptor 3|polymorphism, single nucleotide|nephritis|high-throughput nucleotide sequencing|child