CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis

Yan ZHANG; Sachula WU; Fen-hua LUO; Baiyinbatu; Lin-hong LIU; Tian-yuan HU; Boyang YU; Guang-peng LI; Ying-ji WU

Journal of Integrative Agriculture (Aug 2014)

CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis

Yan ZHANG,
Sachula WU,
Fen-hua LUO,
Baiyinbatu,
Lin-hong LIU,
Tian-yuan HU,
Boyang YU,
Guang-peng LI,
Ying-ji WU

Affiliations

Yan ZHANG: The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot 010021, P.R.China; School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010021, P.R.China
Sachula WU: The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot 010021, P.R.China
Fen-hua LUO: The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot 010021, P.R.China
Baiyinbatu: The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot 010021, P.R.China
Lin-hong LIU: The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot 010021, P.R.China
Tian-yuan HU: The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot 010021, P.R.China
Boyang YU: The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot 010021, P.R.China
Guang-peng LI: The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot 010021, P.R.China
Ying-ji WU: The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot 010021, P.R.China; Correspondence WU Ying-ji, Tel: +86-471-4992443, Mobile: 18686031623

Journal volume & issue: Vol. 13, no. 8
pp. 1759 – 1765

Abstract

Read online

Spermatogonial stem cells (SSCs) are unique stem cells in adult body that can transmit genetic information to the next generation. They have self-renewal potential and can continuously support spermatogenesis throughout life of a male animal. However, the SSC population is extremely small, isolation and purification of the SSCs is challenging, especially for livestock animals. It has been confirmed that CDH1 (cadherin-1, also known as E-cadherin) can be expressed in undifferentiated SSCs of mouse and rats, but it has not been verified in sheep. Here, CDH1 was found as a novel surface marker for sheep SSCs. In this paper, sheep anti-CDH1 polyclonal antibodies were prepared and its activity was checked. Using the obtained antibodies and immunohistochemistry analysis, we confirmed that CDH1 can be expressed by SSCs in sheep testis.

Published in Journal of Integrative Agriculture

ISSN: 2095-3119 (Print); 2352-3425 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Agriculture (General)
Website: https://www.journals.elsevier.com/journal-of-integrative-agriculture

About the journal

Abstract

Keywords