European Journal of Medical Research (Jun 2012)

Common polymorphisms in <it>GSTM1, GSTT1, GSTP1, GSTA1</it> and susceptibility to colorectal cancer in the Central European population

  • Hezova Renata,
  • Bienertova-Vasku Julie,
  • Sachlova Milana,
  • Brezkova Veronika,
  • Vasku Anna,
  • Svoboda Marek,
  • Radová Lenka,
  • Kiss Igor,
  • Vyzula Rostislav,
  • Slaby Ondrej

DOI
https://doi.org/10.1186/2047-783X-17-17
Journal volume & issue
Vol. 17, no. 1
p. 17

Abstract

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Abstract Background Central Europe presents with the highest incidence of sporadic colorectal cancer (CRC) worldwide. As sporadic CRC represents a typical multifactorial disease, it is characterized by intense interaction of the genetic background with the environment. Glutathione S-transferases could act as attractive susceptibility genes for CRC, as they are directly involved in conjugation between glutathione and chemotherapeutics, environmental pollutants and a wide spectrum of xenobiotics. Methods In this study, we investigated associations of polymorphisms in glutathione S-transferases (GSTs) genes, that is GSTA1, GSTT1, GSTM1 and GSTP1, with CRC in a total of 197 cases and 218 controls originating from the Czech Central European population. Polymorphisms were assessed by polymerase chain reaction/restriction fragment length polymorphism-based methods, allele-specific multiplex and allelic discrimination by real-time polymerase chain reaction. Results None of investigated polymorphisms showed any associations with CRC, with the exception of GSTP1; where the heterozygote genotype Ile105Val was associated with decreased risk of CRC (P = 0.043). Conclusions The frequencies observed in our study are in accordance with those from other European Caucasian populations. Based on our studies, examined variability in GST genes is not a major determinant of CRC susceptibility in the Central European population.

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