Specificity and selectivity improvement in doping analysis using comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometry
Samantha S. Barbosa,
Felipe D. Leal,
Monica C. Padilha,
Raphael S. F. Silva,
Henrique Marcelo G. Pereira,
Francisco R. Aquino Neto,
Ademário I. da Silva Júnior
Affiliations
Samantha S. Barbosa
Universidade Federal do Rio de Janeiro
Felipe D. Leal
Universidade Federal do Rio de Janeiro
Monica C. Padilha
Universidade Federal do Rio de Janeiro
Raphael S. F. Silva
Universidade Federal do Rio de Janeiro
Henrique Marcelo G. Pereira
Universidade Federal do Rio de Janeiro
Francisco R. Aquino Neto
Universidade Federal do Rio de Janeiro
Ademário I. da Silva Júnior
Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro
Comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometry was used for the identification of forty doping agents. The improvement in the specificity was remarkable, allowing the resolution of analytes that could not be done by one-dimensional chromatographic systems. The sensitivity observed for different classes of prohibited substances was clearly below the value required by the World Anti-Doping Agency. In addition time-of-flight mass spectrometry gives full spectrum for all analytes without any interference from the matrix, resulting in selectivity improvements. These results could support the implementation of an exhaustive monitoring approach for hundreds of doping agents in a single injection.
