Nature Communications (Mar 2021)

Inhibitory mechanism of reveromycin A at the tRNA binding site of a class I synthetase

  • Bingyi Chen,
  • Siting Luo,
  • Songxuan Zhang,
  • Yingchen Ju,
  • Qiong Gu,
  • Jun Xu,
  • Xiang-Lei Yang,
  • Huihao Zhou

DOI
https://doi.org/10.1038/s41467-021-21902-0
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 10

Abstract

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Reveromycin A (RM-A) selectively inhibits eukaryotic cytoplasmic isoleucyltRNA synthetase (IleRS). Herein, the authors show that RM-A molecule occupies the tRNAIle binding site of Saccharomyces cerevisiae IleRS, and that RM-A cooperates with isoleucine or isoleucyl-adenylate for IleRS binding.