College of Engineering, Huazhong Agricultural University, Wuhan, 430070, China; Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518000, China; Shenzhen Institute of Nutrition and Health, Huazhong Agricultural University, Wuhan, 430070, China; Key Laboratory of Agricultural Equipment in Mid-Lower Yangtze River, Ministry of Agriculture and Rural Affairs, Wuhan, 430070, China; The Center of Crop Nanobiotechnology, Huazhong Agricultural University, Wuhan, 430070, China; Corresponding author. College of Engineering, Huazhong Agricultural University, Wuhan, 430070, China.
Zhihan Xu
College of Engineering, Huazhong Agricultural University, Wuhan, 430070, China
Chao Yi
College of Engineering, Huazhong Agricultural University, Wuhan, 430070, China
Yuankai Zhang
College of Engineering, Huazhong Agricultural University, Wuhan, 430070, China
Qingxi Liao
College of Engineering, Huazhong Agricultural University, Wuhan, 430070, China; Key Laboratory of Agricultural Equipment in Mid-Lower Yangtze River, Ministry of Agriculture and Rural Affairs, Wuhan, 430070, China; Corresponding author. College of Engineering, Huazhong Agricultural University, Wuhan, 430070, China.
Carbendazim and acetamidine are pesticides that widely used to control pests and diseases in oilseed rape. In this paper, a rapid, accurate and reliable method was proposed for the detection of carbendazim and acetamidine with SERS microfluidic chip technology. Ag-ps(Polystyrene microspheres) microsphere SERS substrate was prepared by spin coating and magnetron sputtering deposition of Ag. The enhancement factor of prepared SERS substrate was 2.4 × 1010. The SERS detection working curves were well fitted and the linear parameters R2 were 0.987 and 0.994, respectively. The limit of detection was 0.01 mg/mL. The use of SERS microfluidic chip to detect carbendazim and acetamidine is expected to provide a way for the detection of pesticide residues in crops, which has broad application prospects in the field of food safety.
