Nature Communications (Jul 2018)
Organoid cultures recapitulate esophageal adenocarcinoma heterogeneity providing a model for clonality studies and precision therapeutics
- Xiaodun Li,
- Hayley E. Francies,
- Maria Secrier,
- Juliane Perner,
- Ahmad Miremadi,
- Núria Galeano-Dalmau,
- William J. Barendt,
- Laura Letchford,
- Genevieve M. Leyden,
- Emma K. Goffin,
- Andrew Barthorpe,
- Howard Lightfoot,
- Elisabeth Chen,
- James Gilbert,
- Ayesha Noorani,
- Ginny Devonshire,
- Lawrence Bower,
- Amber Grantham,
- Shona MacRae,
- Nicola Grehan,
- David C. Wedge,
- Rebecca C. Fitzgerald,
- Mathew J. Garnett
Affiliations
- Xiaodun Li
- MRC Cancer Unit, University of Cambridge
- Hayley E. Francies
- Wellcome Sanger Institute, Hinxton
- Maria Secrier
- Cancer Research UK Cambridge Institute
- Juliane Perner
- Cancer Research UK Cambridge Institute
- Ahmad Miremadi
- Cambridge University Hospitals NHS Trust
- Núria Galeano-Dalmau
- MRC Cancer Unit, University of Cambridge
- William J. Barendt
- Wellcome Sanger Institute, Hinxton
- Laura Letchford
- Wellcome Sanger Institute, Hinxton
- Genevieve M. Leyden
- Wellcome Sanger Institute, Hinxton
- Emma K. Goffin
- Wellcome Sanger Institute, Hinxton
- Andrew Barthorpe
- Wellcome Sanger Institute, Hinxton
- Howard Lightfoot
- Wellcome Sanger Institute, Hinxton
- Elisabeth Chen
- Wellcome Sanger Institute, Hinxton
- James Gilbert
- Wellcome Sanger Institute, Hinxton
- Ayesha Noorani
- MRC Cancer Unit, University of Cambridge
- Ginny Devonshire
- Cancer Research UK Cambridge Institute
- Lawrence Bower
- Cancer Research UK Cambridge Institute
- Amber Grantham
- MRC Cancer Unit, University of Cambridge
- Shona MacRae
- MRC Cancer Unit, University of Cambridge
- Nicola Grehan
- Cambridge University Hospitals NHS Trust
- David C. Wedge
- Big Data Institute, University of Oxford
- Rebecca C. Fitzgerald
- MRC Cancer Unit, University of Cambridge
- Mathew J. Garnett
- Wellcome Sanger Institute, Hinxton
- DOI
- https://doi.org/10.1038/s41467-018-05190-9
- Journal volume & issue
-
Vol. 9,
no. 1
pp. 1 – 13
Abstract
Esophageal adenocarcinoma (EAC) has a poor 5-year survival rate and lacks robust preclinical models for use in research. Here, the authors show that newly derived organoids recapitulate the transcriptomic, genetic, and morphological landscape of the primary EAC tumors and provide a platform to test drug sensitivity and study tumor clonality.
