Copper oxide nanoparticles induces oxidative stress and liver toxicity in rats following oral exposure

Abstract

The present study aimed to evaluate the effect of Copper Oxide (CuO) Nanoparticles (NP) on liver following oral exposure in rats. A total of 18 male wister rats were used in the experiments including a control group (6 rats). NP were given to the rats with a doses (5, 50 mg/kg b.w./day) via oral gavage and a control group (received only 200 μl PBS). The treatment was continued for 14 days. The supernatants of rat Liver tissue homogenates were used to analyze for glutathione levels (GSH), Catalase, superoxide dismutase (SOD), the extent of lipid peroxidation products (Malondialdehyde, MDA). Oral administration of NP to rats caused a significant (P < 0.05) dose dependent alterations in antioxidant enzyme activities. Data results clearly showed the significant decrease (p < 0.05) in GSH, Catalase (CAT) and SOD activity, whereas the lipid peroxidation product (MDA) levels were increased (p < 0.05). In conclusion, oral exposure of CuO nanoparticles to rats causes significant toxicity to the liver and it might be due to oxidative stress. Keywords: Copper oxide nanoparticles, CuO, NP, Liver toxicity, GSH, CAT, SOD, MDA