CHARACTERISTICS OF CYTOKINE PROFILE LEVEL (TNFα, IL-6, IL-10) AND THEIR SOLUBLE RECEPTORS (SRр55 TNFα, SRр75 TNFα, SR IL-6) IN THE PATIENTS WITH HIV, HIV-ASSOCIATED TUBERCULOSIS AND PULMONARY TUBERCULOSIS

Abstract

Abstract. Three groups of patients were investigated as follows: 1st group, HIV-infected patients, 2nd group, patients with HIV-associated tuberculosis, the 3rd group, persons with pulmonary tuberculosis. The amounts of cytokines (TNFα, IL-6, IL-10) and their soluble receptors (SRp55 TNFα, SRp75 TNFα, SR IL-6) were evaluated in blood serum by solid-phase immuno-enzyme technique. The patients did not receive antiviral therapy before, or during the investigations. Some features are revealed reflecting a direction and significance of changes in immune reactivity of the patients. In the patients with tuberculosis, IL-10 and IL-6 levels were increased, along with pronounced rise of srIL-6, relatively low index of SRp75 TNFα and deficiency of SRp55 TNFα, associated with moderate increase in TNFα. In the patients with HIV and HIV-associated tuberculosis, the direction of alterations were found to be similar, except of IL-10, i.e., a hyper-production of TNFα and its soluble type I and II receptors, increased IL-6 level in combination with lack of its soluble receptor. Correlation analysis showed a strict dependence between TNFα and viral load in the patients with IVB and V stage of HIV, and HIV-associated tuberculosis that argues for significance of TNFα in pathogenesis of HIV-infection and its progression. We have found differential trends in dynamics of cytokines under study and their soluble receptors in the course of HIV-infection progression in the groups I and II of patients, that allowing us of elaborating additional diagnostic criteria of HIV-associated tuberculosis. (Med. Immunol., vol. 10, N 6, pp 519-526).

Keywords

• cytokines
• soluble cytokines receptors
• hiv-infected patients
• hiv-associated tuberculosis