Activity of Compounds from Temperate Propolis against Trypanosoma brucei and Leishmania mexicana

Adullah Alotaibi; Godwin U. Ebiloma; Roderick Williams; Ibrahim A. Alfayez; Manal J. Natto; Sameah Alenezi; Weam Siheri; Malik AlQarni; John O. Igoli; James Fearnley; Harry P. De Koning; David G. Watson

doi:10.3390/molecules26133912

Molecules (Jun 2021)

Activity of Compounds from Temperate Propolis against Trypanosoma brucei and Leishmania mexicana

Adullah Alotaibi,
Godwin U. Ebiloma,
Roderick Williams,
Ibrahim A. Alfayez,
Manal J. Natto,
Sameah Alenezi,
Weam Siheri,
Malik AlQarni,
John O. Igoli,
James Fearnley,
Harry P. De Koning,
David G. Watson

Affiliations

Adullah Alotaibi: Strathclyde Institute of Pharmacy and Biomedical Science, University of Strathclyde, 161 Cathedral Street, Glasgow G4 0RE, UK
Godwin U. Ebiloma: Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8TA, UK
Roderick Williams: IBEHR, School of Health and Life Science, University of the West of Scotland, High Street, Paisley PA1 2BE, UK
Ibrahim A. Alfayez: Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8TA, UK
Manal J. Natto: Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8TA, UK
Sameah Alenezi: Strathclyde Institute of Pharmacy and Biomedical Science, University of Strathclyde, 161 Cathedral Street, Glasgow G4 0RE, UK
Weam Siheri: Department of Pharmacognosy and Natural Products, Faculty of Pharmacy, University of Tripoli, Tripoli 50676, Libya
Malik AlQarni: Department of Pharmaceutical Chemistry, College of Clinical Pharmacy, Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi Arabia
John O. Igoli: Strathclyde Institute of Pharmacy and Biomedical Science, University of Strathclyde, 161 Cathedral Street, Glasgow G4 0RE, UK
James Fearnley: BeeVital, Whitby, North Yorkshire YO22 5JR, UK
Harry P. De Koning: Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8TA, UK
David G. Watson: Strathclyde Institute of Pharmacy and Biomedical Science, University of Strathclyde, 161 Cathedral Street, Glasgow G4 0RE, UK

DOI: https://doi.org/10.3390/molecules26133912
Journal volume & issue: Vol. 26, no. 13
p. 3912

Abstract

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Ethanolic extracts of samples of temperate zone propolis, four from the UK and one from Poland, were tested against three Trypanosoma brucei strains and displayed EC50 values T. brucei and Leishmania mexicana, with the highest activities ≤ 15 µM. The most active compounds against T. brucei were naringenin 4′,7 dimethyl ether and 4′methoxy kaempferol with activity of 15–20 µM against the three T. brucei strains. The most active compounds against L. mexicana were 4′,7-dimethoxykaempferol and the coumaric acid ester mixture, with EC50 values of 12.9 ± 3.7 µM and 13.1 ± 1.0 µM. No loss of activity was found with the diamidine- and arsenical-resistant or phenanthridine-resistant T. brucei strains, or the miltefosine-resistant L. mexicana strain; no clear structure activity relationship was observed for the isolated compounds. Temperate propolis yields multiple compounds with anti-kinetoplastid activity.

Published in Molecules

ISSN: 1420-3049 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/molecules

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