Using a simple spectrophotometer to analyze cypress hydrolat composition

Abstract

The Pure Dew (Cypress Hydrolat), which could be extracted from the waste material after the extracting essential oil from Taiwan cypress, has a good bactericidal effect. However, due to the high cost on quality control and concentration measurement of the Pure Dew, its application was restricted. This research tries to find suitable spectral frequencies through which the absorbance detected by the spectrometer could be used as the index of the pure dew concentration. This study used Gas Chromatography-Mass Spectrophotometer (GC-MS) to analyze the composition of Taiwan cypress hydrolat. After obtaining the composition, the raw liquor of cypress hydrolat was diluted to 100, 50, 25 and 0% v/v with pure water. The test samples were then tested by a simple spectrophotometer. After the spectrographic detection of absorbance using a simple spectrophotometer, it is confirmed that the spectrum of wavelength between 205–350 nm is the most representative. The absorptance and the pure dew concentration was roughly in linear relation which suggested that a simple spectrophotometer can be used to develop a low-cost and high.

Keywords

• cypress hydrolat
• gc-ms
• spectrophotometer
• concentration measurement