A redox-mediated Kemp eliminase

Aitao Li; Binju Wang; Adriana Ilie; Kshatresh D. Dubey; Gert Bange; Ivan V. Korendovych; Sason Shaik; Manfred T. Reetz

doi:10.1038/ncomms14876

Nature Communications (Mar 2017)

A redox-mediated Kemp eliminase

Aitao Li,
Binju Wang,
Adriana Ilie,
Kshatresh D. Dubey,
Gert Bange,
Ivan V. Korendovych,
Sason Shaik,
Manfred T. Reetz

Affiliations

Aitao Li: Department of Biocatalysis, Max-Planck-Institut für Kohlenforschung
Binju Wang: Institute of Chemistry and the Lise Meitner-Minerva Center for Computational Quantum Chemistry, The Hebrew University of Jerusalem
Adriana Ilie: Department of Biocatalysis, Max-Planck-Institut für Kohlenforschung
Kshatresh D. Dubey: Institute of Chemistry and the Lise Meitner-Minerva Center for Computational Quantum Chemistry, The Hebrew University of Jerusalem
Gert Bange: LOEWE Center for Synthetic Microbiology (SYNMIKRO) and Department of Chemistry, Philipps-Universität Marburg
Ivan V. Korendovych: Department of Chemistry, Syracuse University
Sason Shaik: Institute of Chemistry and the Lise Meitner-Minerva Center for Computational Quantum Chemistry, The Hebrew University of Jerusalem
Manfred T. Reetz: Department of Biocatalysis, Max-Planck-Institut für Kohlenforschung

DOI: https://doi.org/10.1038/ncomms14876
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 8

Abstract

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The majority of enzymatic Kemp elimination reactions proceed via a well-established acid-base mechanism. Here, the authors show that cytochrome P450 is able to metabolize the leflunomide drug via a redox Kemp elimination, offering new insights into enzyme catalysis.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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