Pathogen spectrum analysis and key risk control of donated human milk in human milk bank

Abstract

Objective To make pathogen spectrum analysis of donor human milk (DHM) in human milk bank and investigate the risk control in the operation of human milk bank. Methods Liquid hyperplasia culture was used for microbiological detection of DHM samples in the human milk bank of our hospital. A total of 1 601 DHM samples in the human milk bank of our hospital from December 2015 to December 2021 were selected. The samples were randomly divided into pre-pasteurization group (n=866) and post-pasteurization group (n=735). The results of microbiological detection of the 2 groups of samples were further analyzed with pathogen spectrum analysis. The results of environmental hygiene test for the human milk bank from 2015 to 2021 were selected and trend analysis was applied to analyze the effectiveness of environmental infection control. Results Before pasteurization, 74.36% of the DHM samples cultured with pathogens, and 25.64% of them were without pathogens. After pasteurization, 12.52% of the DHM samples had pathogens and 87.48% did not. The pathogen detection rate was significantly lower after pasteurization than before disinfection (P < 0.05); After disinfection, the detection rate of pathogenic bacteria was decreased with statistical significance (P < 0.05). Two kinds of anaerobic bacteria were detected in the DHM samples after anaerobic and aerobic culture: Clostridium perfringens and Clostridium tertiary. The qualified rate of environmental hygiene monitoring was increased year by year in the human milk bank (P < 0.05). Conclusion Pasteurization can not completely kill the pathogen in the DHM samples. The microbiological detection of the DHM by liquid enrichment culture can improve the pathogen detection rate, and the standardized environmental cleaning and disinfection can improve the qualified rate of environmental health detection in the human milk bank. It is the key risk control point to guarantee the safe operation of the human milk bank.

Keywords

• human milk bank
• pathogenic spectrum analysis of donated human milk
• risk control