Gene sequence diversity of the nonribosomal peptide and polyketide natural products in Changbaishan soil correlates with changes in landscape belts

Haipeng Zhang; Yuehua Liu; Xinhai Wang; Renge Hu; Guanyi Xu; Changsi Mao; Muhammad Inam; Yiming Wang; Xiuyun Jiang; Lingcong Kong; Hongxia Ma

Ecological Indicators (Dec 2021)

Gene sequence diversity of the nonribosomal peptide and polyketide natural products in Changbaishan soil correlates with changes in landscape belts

Haipeng Zhang,
Yuehua Liu,
Xinhai Wang,
Renge Hu,
Guanyi Xu,
Changsi Mao,
Muhammad Inam,
Yiming Wang,
Xiuyun Jiang,
Lingcong Kong,
Hongxia Ma

Affiliations

Haipeng Zhang: College of Life Science, Jilin Agricultural University, Xincheng Street No. 2888, Changchun 130118, China; The Engineering Research Center of Bioreactor and Drug Development, Ministry of Education, Jilin Agricultural University, Xincheng Street No. 2888, Changchun 130118, China
Yuehua Liu: College of Life Science, Jilin Agricultural University, Xincheng Street No. 2888, Changchun 130118, China; The Engineering Research Center of Bioreactor and Drug Development, Ministry of Education, Jilin Agricultural University, Xincheng Street No. 2888, Changchun 130118, China
Xinhai Wang: College of Veterinary Medicine, Jilin Agricultural University, Xincheng Street No. 2888, Changchun 130118, China
Renge Hu: Marine College, Shandong University, Weihai, Shandong 264209, China
Guanyi Xu: College of Veterinary Medicine, Jilin Agricultural University, Xincheng Street No. 2888, Changchun 130118, China
Changsi Mao: Veterinary Medicine, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian, Beijing 100193, China
Muhammad Inam: College of Veterinary Medicine, Jilin Agricultural University, Xincheng Street No. 2888, Changchun 130118, China
Yiming Wang: College of Veterinary Medicine, Jilin Agricultural University, Xincheng Street No. 2888, Changchun 130118, China
Xiuyun Jiang: College of Life Science, Jilin Agricultural University, Xincheng Street No. 2888, Changchun 130118, China; Changchun Sci-Tech University, Shuangyang District, Changchun 130600, China
Lingcong Kong: College of Veterinary Medicine, Jilin Agricultural University, Xincheng Street No. 2888, Changchun 130118, China; The Key Laboratory of New Veterinary Drug Research and Development of Jilin Province, Jilin Agricultural University, Xincheng Street No. 2888, Changchun 130118, China; Corresponding authors at: College of Veterinary Medicine, Jilin Agricultural University, Xincheng Street No. 2888, Changchun 130118, China, Jilin Agricultural University, Xincheng Street No. 2888, Changchun 130118, China (L. Kong); College of Life Science, Jilin Agricultural University, Xincheng Street No.2888, Changchun 130118, China (H. Ma).
Hongxia Ma: College of Life Science, Jilin Agricultural University, Xincheng Street No. 2888, Changchun 130118, China; The Engineering Research Center of Bioreactor and Drug Development, Ministry of Education, Jilin Agricultural University, Xincheng Street No. 2888, Changchun 130118, China; The Key Laboratory of New Veterinary Drug Research and Development of Jilin Province, Jilin Agricultural University, Xincheng Street No. 2888, Changchun 130118, China; Corresponding authors at: College of Veterinary Medicine, Jilin Agricultural University, Xincheng Street No. 2888, Changchun 130118, China, Jilin Agricultural University, Xincheng Street No. 2888, Changchun 130118, China (L. Kong); College of Life Science, Jilin Agricultural University, Xincheng Street No.2888, Changchun 130118, China (H. Ma).

Journal volume & issue: Vol. 133
p. 108160

Abstract

Read online

Nonribosomal peptides (NRPs) and polyketides (PKs) have widely been widely applied in clinical drugs in use today, and one of the main sources for novel NRPs and PKs is soil microbes. However, the diversity of soil bacteria and distribution of their NRPs and PKs biosynthetic genes in different landscapes in extreme environments have not been reported. In this study, 16S rRNA gene fragments from five landscape belts in Changbaishan were analyzed. The results demonstrated that Acidobacteria, Proteobacteria and Chloroflexi accounted for 65% of all bacterial species. Furthermore, the diversities of the PKs and NRPs in the different landscape belts of Changbaishan were compared based on the diversity of gene fragments of the polyketide synthase (PKs) ketosynthase (KS) domain and nonribosomal peptide synthetase (NRPs) adenylation domain (AD). The results showed that NRPs and PKs had highest richness in alpine tundra and birch forest belts, which were negatively correlated with the contents of the available phosphorus, nitrogen, calcium, potassium, and soil organic matter. NRPs and PKs mainly existed in unidentified bacteria, Proteobacteria and Actinobacteria. In addition, a 16S rRNA KEGG pathway analysis showed that the soil microorganisms of Changbaishan have the potential to promote secondary metabolite biosynthesis, resist diseases and regulate immune systems. These combined results suggest that soils of alpine tundra and birch forest belts in Changbaishan may be the main sources for searching the novel antibacterial drugs.

Published in Ecological Indicators

ISSN: 1470-160X (Print); 1872-7034 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General): Ecology
Website: https://www.sciencedirect.com/journal/ecological-indicators

About the journal

Abstract

Keywords