AFM and MFM techniques for enzyme activity imaging and quantification

Abstract

In this work, a new approach for enzyme activity monitoring is suggested. It is based on the real-time imaging, by atomic force microscopy (AFM) and magnetic force microscopy (MFM), of the degradation of a nanoparticles-loaded enzyme responsive layer, namely Fe3O4-nanoparticles-loaded gelatin. The roughness analysis allowed the quantification of the trypsin-induced ferrogel degradation and the correlation of the AFM and MFM data obtained.

Keywords

• Atomic force microscopy
• magnetic force microscopy
• trypsin activity
• ferrogel