Diacylglycerol Acetyltransferase Gene Isolated from <i>Euonymus europaeus</i> L. Altered Lipid Metabolism in Transgenic Plant towards the Production of Acetylated Triacylglycerols
Daniel Mihálik,
Andrea Lančaričová,
Michaela Mrkvová,
Šarlota Kaňuková,
Jana Moravčíková,
Miroslav Glasa,
Zdeno Šubr,
Lukáš Predajňa,
Richard Hančinský,
Simona Grešíková,
Michaela Havrlentová,
Pavol Hauptvogel,
Ján Kraic
Affiliations
Daniel Mihálik
Research Institute of Plant Production, National Agricultural and Food Center, Bratislavská cesta 122, 92168 Piešt’any, Slovakia
Andrea Lančaričová
Research Institute of Plant Production, National Agricultural and Food Center, Bratislavská cesta 122, 92168 Piešt’any, Slovakia
Michaela Mrkvová
Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Námestie J. Herdu 2, 91701 Trnava, Slovakia
Šarlota Kaňuková
Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Námestie J. Herdu 2, 91701 Trnava, Slovakia
Jana Moravčíková
Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Námestie J. Herdu 2, 91701 Trnava, Slovakia
Miroslav Glasa
Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Námestie J. Herdu 2, 91701 Trnava, Slovakia
Zdeno Šubr
Institute of Virology, Biomedical Research Centre, Slovak Academy of Sciences, Dúbravská cesta 9, 84505 Bratislava, Slovakia
Lukáš Predajňa
Institute of Virology, Biomedical Research Centre, Slovak Academy of Sciences, Dúbravská cesta 9, 84505 Bratislava, Slovakia
Richard Hančinský
Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Námestie J. Herdu 2, 91701 Trnava, Slovakia
Simona Grešíková
Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Námestie J. Herdu 2, 91701 Trnava, Slovakia
Michaela Havrlentová
Research Institute of Plant Production, National Agricultural and Food Center, Bratislavská cesta 122, 92168 Piešt’any, Slovakia
Pavol Hauptvogel
Research Institute of Plant Production, National Agricultural and Food Center, Bratislavská cesta 122, 92168 Piešt’any, Slovakia
Ján Kraic
Research Institute of Plant Production, National Agricultural and Food Center, Bratislavská cesta 122, 92168 Piešt’any, Slovakia
Euonymus species from the Celastraceae family are considered as a source of unusual genes modifying the oil content and fatty acid composition of vegetable oils. Due to the possession of genes encoding enzyme diacylglycerol acetyltransferase (DAcT), Euonymus plants can synthesize and accumulate acetylated triacyglycerols. The gene from Euonymus europaeus (EeDAcT) encoding the DAcT was identified, isolated, characterized, and modified for cloning and genetic transformation of plants. This gene has a unique nucleotide sequence and amino acid composition, different from orthologous genes from other Euonymus species. Nucleotide sequence of original EeDAcT gene was modified, cloned into transformation vector, and introduced into tobacco plants. Overexpression of EeDAcT gene was confirmed, and transgenic host plants produced and accumulated acetylated triacylglycerols (TAGs) in immature seeds. Individual transgenic plants showed difference in amounts of synthesized acetylTAGs and also in fatty acid composition of acetylTAGs.
