Virology Journal (Oct 2009)

Phylogenetic analysis and characterization of Korean orf virus from dairy goats: case report

  • Jean Young-Hwa,
  • Kim Hye-Ryoung,
  • Lee Kyoung-Ki,
  • Lee Kyung-Hyun,
  • Roh In-Soon,
  • Oem Jae-Ku,
  • Lee O-Soo

DOI
https://doi.org/10.1186/1743-422X-6-167
Journal volume & issue
Vol. 6, no. 1
p. 167

Abstract

Read online

Abstract An outbreak of orf virus infection in dairy goats in Korea was investigated. Suspected samples of the skin and lip of affected goats were sent to the laboratory for more exact diagnosis. Orf virus was detected by electron microscopy and viral DNA was identified by PCR. To reveal the genetic characteristics of the Korean strain (ORF/09/Korea), the sequences of the major envelope protein (B2L) and orf virus interferon resistance (VIR) genes were determined and then compared with published reference sequences. Phylogenetic analysis revealed that the ORF/09/Korea strain was closest to the isolates (Taiping) from Taiwan. This is believed to be the first report on the molecular characterization of orf virus in Korea.