Light-emitting diode-based absorbance detectors for flow-through analysis in analytical chemistry: A tutorial

Abstract

The development of light-emitting diodes (LEDs) has played a significant role supporting many recent advances made in chemical analysis. Their small size, low power consumption, and semi-monochromaticity make them ideal light sources for portable photometric devices used in field and on-site analysis, environmental monitoring, and industrial applications, such as process analytical technology (PAT) and continuous real-time analysis. This tutorial provides an overview and critical comparison of the key components in LED-based absorbance detectors, including light sources, optical fibres, flow cells, and photodetectors. Fundamental aspects of LEDs relating to their use in such simple detectors are discussed in detail, along with the development of multi-wavelength detectors that incorporate multiple LEDs. The tutorial also details the different methodologies for assessing and characterising the LED-based absorbance detectors. Finally, this tutorial presents some selected applications of LED based photometric detectors in gas sensing, flow injection analysis (FIA), and coupled with separation techniques such as ion chromatography, liquid chromatography, and capillary zone electrophoresis (CZE).

Keywords

• Light emitting diodes
• UV–Vis absorbance detectors
• Multi-wavelength absorbance detection
• Flow-through analysis
• Portable LED-based instrumentation