Brazilian Journal of Medical and Biological Research (Aug 2002)

Genetic characterization of dengue virus type 3 isolates in the State of Rio de Janeiro, 2001

  • M.P. Miagostovich,
  • F.B. dos Santos,
  • T.S. de Simone,
  • E.V. Costa,
  • A.M.B. Filippis,
  • H.G. Schatzmayr,
  • R.M.R. Nogueira

DOI
https://doi.org/10.1590/S0100-879X2002000800002
Journal volume & issue
Vol. 35, no. 8
pp. 869 – 872

Abstract

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The genetic characterization of dengue virus type 3 (DEN-3) strains isolated from autochthonous cases in the State of Rio de Janeiro, Brazil, in 2001 is presented. Restriction site-specific (RSS)-PCR performed on 22 strains classified the Brazilian DEN-3 viruses as subtype C, a subtype that contains viruses from Sri Lanka, India, Africa and recent isolates from Central America. Nucleic acid sequencing (positions 278 to 2550) of one DEN-3 strain confirmed the origin of these strains, since genotype III - classified by sequencing - and RSS-PCR subtype C are correlated. This genetic subtype has been associated with hemorrhagic dengue epidemics and the information provided here could be useful to implement appropriate prevention and control measures.

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