In vitro effects of culture media on potential regeneration of date palm (Phoenix dactylifera L.) in Boussaâda oasis, Algeria

Abstract

Date palm (Phoenix dactylifera L.) of Boussaâda has varietal diversity and a very important nutritional value in Algeria. Nevertheless, today the palm groves are threatened, not only climatically but also in terms of varietal diversity such as disappearance of varieties, the low production and even discharges serving mainly to vegetative propagation which can be used as a source of explants on in vitro somatic embryogenesis technique. The aim of this study is to use in vitro culture techniques to regenerate the phoenicicultural orchards at Boussaâda oasis. Two types of experimental protocol are used; firstly, to make an inventory in field in order to contain palm groves and then date palms existing; and the secondly is to applicant the somatic embryogenesis technique in the laboratory and then regenerate the good cultivar. Results indicate that plant growing is an important axis that needs to be developed in the oasis by introducing varieties to select the most suitable and profitable for a good diversification of cultivars and consequently the improvement of production.