Enhancing the Antimicrobial Effect of Ozone with <i>Mentha piperita</i> Essential Oil
Alin-Daniel Floare,
Ramona Dumitrescu,
Vlad Tiberiu Alexa,
Octavia Balean,
Camelia Szuhanek,
Diana Obistioiu,
Ileana Cocan,
Alina-Georgeta Neacsu,
Iuliana Popescu,
Aurora Doris Fratila,
Atena Galuscan
Affiliations
Alin-Daniel Floare
Translational and Experimental Clinical Research Centre in Oral Health, University of Medicine and Pharmacy “Victor Babes”, Eftimie Murgu Sq. no 2, 300041 Timisoara, Romania
Ramona Dumitrescu
Translational and Experimental Clinical Research Centre in Oral Health, University of Medicine and Pharmacy “Victor Babes”, Eftimie Murgu Sq. no 2, 300041 Timisoara, Romania
Vlad Tiberiu Alexa
Translational and Experimental Clinical Research Centre in Oral Health, University of Medicine and Pharmacy “Victor Babes”, Eftimie Murgu Sq. no 2, 300041 Timisoara, Romania
Octavia Balean
Translational and Experimental Clinical Research Centre in Oral Health, University of Medicine and Pharmacy “Victor Babes”, Eftimie Murgu Sq. no 2, 300041 Timisoara, Romania
Camelia Szuhanek
Department of Preventive, Community Dentistry and Oral Health, University of Medicine and Pharmacy “Victor Babes”, Eftimie Murgu Sq. no 2, 300041 Timisoara, Romania
Diana Obistioiu
Faculty of Veterinary Medicine, University of Life Sciences “King Mihai I” from Timisoara, Calea Aradului No. 119, 300641 Timisoara, Romania
Ileana Cocan
Faculty of Food Engineering, University of Life Sciences “King Mihai I” from Timisoara, Calea Aradului No. 119, 300645 Timisoara, Romania
Alina-Georgeta Neacsu
Faculty of Agriculture, University of Life Sciences “King Mihai I” from Timisoara, Calea Aradului No. 119, 300641 Timisoara, Romania
Iuliana Popescu
Faculty of Agriculture, University of Life Sciences “King Mihai I” from Timisoara, Calea Aradului No. 119, 300641 Timisoara, Romania
Aurora Doris Fratila
Faculty of Dental Medicine, Ludwig-Maximilian University Munich, Goethestr. 70, 80336 Munich, Germany
Atena Galuscan
Translational and Experimental Clinical Research Centre in Oral Health, University of Medicine and Pharmacy “Victor Babes”, Eftimie Murgu Sq. no 2, 300041 Timisoara, Romania
This study aimed to obtain and analyse Mentha piperita essential oil (MpEO) for the prospect of being used as an enhancement agent for the antimicrobial potential of ozone against gram-positive and gram-negative bacteria and fungi. The research was done for different exposure times, and it gained time–dose relationships and time–effect correlations. Mentha piperita (Mp) essential oil (MpEO) was obtained via hydrodistillation and further analysed by using GC-MS. The broth microdilution assay was used to determine the strain inhibition/strain mass growth by using spectrophotometric optical density reading (OD). The bacterial/mycelium growth rates (BGR/MGR) and the bacterial/mycelium inhibition rates (BIR/MIR) after ozone treatment in the presence and absence of MpEO on the ATTC strains were calculated; the minimum inhibition concentration (MIC) and statistical interpretations of the time–dose relationship and specific t-test correlations were determined. The effect of ozone on the following tested strains at maximum efficiency was observed after 55 s of single ozone exposure, in order of effect strength: S. aureus > P. aeruginosa > E. coli > C. albicans > S. mutans. For ozone with the addition of 2% MpEO (MIC), maximum efficacy was recorded at 5 s for these strains, in order of effect strength: C. albicans > E. coli > P. aeruginosa > S. aureus > S. mutans. The results suggest a new development and affinity regarding the cell membrane of the different microorganisms tested. In conclusion, the use of ozone, combined with MpEO, is sustained as an alternative therapy in plaque biofilm and suggested as helpful in controlling oral disease-causing microorganisms in medicine.
