Generation of ZZUi008-A, a transgene-free, induced pluripotent stem cell line derived from chorionic villi cells of a fetus with Duchenne muscular dystrophy

Xiaofan Zhu; Aojie Cai; Jingjing Meng; Lina Liu; Siying Cui; Suzhen Qu; Junhong Zhao; Ning Liu; Xiangdong Kong

Stem Cell Research (Oct 2018)

Generation of ZZUi008-A, a transgene-free, induced pluripotent stem cell line derived from chorionic villi cells of a fetus with Duchenne muscular dystrophy

Xiaofan Zhu,
Aojie Cai,
Jingjing Meng,
Lina Liu,
Siying Cui,
Suzhen Qu,
Junhong Zhao,
Ning Liu,
Xiangdong Kong

Affiliations

Xiaofan Zhu: Genetics and Prenatal Diagnosis Center, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Rd, Erqi District, Zhengzhou, Henan Province, China
Aojie Cai: Genetics and Prenatal Diagnosis Center, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Rd, Erqi District, Zhengzhou, Henan Province, China
Jingjing Meng: Genetics and Prenatal Diagnosis Center, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Rd, Erqi District, Zhengzhou, Henan Province, China
Lina Liu: Genetics and Prenatal Diagnosis Center, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Rd, Erqi District, Zhengzhou, Henan Province, China
Siying Cui: Genetics and Prenatal Diagnosis Center, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Rd, Erqi District, Zhengzhou, Henan Province, China
Suzhen Qu: Genetics and Prenatal Diagnosis Center, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Rd, Erqi District, Zhengzhou, Henan Province, China
Junhong Zhao: Genetics and Prenatal Diagnosis Center, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Rd, Erqi District, Zhengzhou, Henan Province, China
Ning Liu: Genetics and Prenatal Diagnosis Center, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Rd, Erqi District, Zhengzhou, Henan Province, China
Xiangdong Kong: Corresponding author.; Genetics and Prenatal Diagnosis Center, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Rd, Erqi District, Zhengzhou, Henan Province, China

Journal volume & issue: Vol. 32
pp. 47 – 50

Abstract

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Duchenne muscular dystrophy (DMD) is a common X-linked recessive disorder for which there is no present cure. In this paper, we reported the generation of ZZUi008-A, an induced pluripotent stem cell(iPSC) line derived from chorionic villus(CV) cells of a fetus with a deletion mutation in exon 33 of the dystrophin gene (DMD). The cell line was generated using feeder-free and virus-free conditions, and the established cell line retains the original DMD mutation, a normal karyotype, expresses pluripotency markers, able to differentiate into three lineages. This ZZUi008-A cell line could provide a promising tool to study this complex disease.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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