Revista do Instituto de Medicina Tropical de São Paulo (Jan 2003)

Molecular typing of dengue virus type 2 in Brazil

  • Marize P. Miagostovich,
  • Patrícia C. Sequeira,
  • Flávia B. dos Santos,
  • Amanda Maia,
  • Rita Maria R. Nogueira,
  • Hermann G. Schatzmayr,
  • Eva Harris,
  • Lee W. Riley

Journal volume & issue
Vol. 45, no. 1
pp. 17 – 21

Abstract

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Strain typing is a critical tool for molecular epidemiological analysis and can provide important information about the spread of dengue viruses. Here, we performed a molecular characterization of DEN-2 viruses isolated in Brazil during 1990-2000 from geographically and temporally distinct areas in order to investigate the genetic distribution of this serotype circulating in the country. Restriction site-specific polymerase chain reaction (RSS)-PCR presented the same pattern for all 52 Brazilian samples, showing the circulation of just one DEN-2 variant. Phylogenetic analysis using progressive pairwise alignments from 240-nucleotide sequences of the E/NS1 junction in 15 isolates showed that they belong to genotype III (Jamaica genotype).

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