Revista MVZ Cordoba (Dec 2008)

Analysis of two populations of the Uruguayan canine breed Cimarron (Canis familiaris) using rapd markers

  • Silvia Llambí D,
  • Rosa Gagliardi B,
  • Mónica Martínez T,
  • Jorge Estevez C,
  • Ana Gorozurreta O,
  • María Victoria Arruga L

Journal volume & issue
Vol. 13, no. 3
pp. 1464 – 1468

Abstract

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Objective. To analyze the genetic variability in two populations of the native canine breed “Uruguayan Cimarron” from different areas of the country. Materials and methods. DNA was isolated from 40 animals of the Cimarron breed. Using PCR, 11 RAPD (random amplified polymorphism of DNA) molecular markers were analyzed. Results. 14.9% of loci were polymorphic. We found an index of shared bands of 0.91, a genetic distance of 0.16 and a Nei’s average diversity index of 0.08. Conclusions. Both populations present a high homogeneity and genetic identity for these markers suggesting that they belong to a common genetic nucleus.

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