Effects of 4 kinds of dental metal alloy extraction on expression of inflanmatory cytokine in the inflamed periodontal tissue

Abstract

Objective To evaluate the inflammatory cytokine expressions of inflamed human gingival fibroblast cells (iHGFs) stimulated in 4 types of dental metal extractions.Methods Inflamed gingival tissues from 10 patients with periodontitis were collected, and primary iHGFs were cultured. The cells were stimulated by Co-Cr, Ti alloy, pure Ti and Au-Pd alloy extractions respectively, and DMEM was used as blank control. Protein productions of pro-inflammatory mediators and related mRNA expressions were measured with ELISA and RT-PCR. Statistics were tested with Wilcoxon matched-pairs test.Results ELISA and RT-PCR results showed the protein and mRNA expressions of interleukin-6, interleukin-1β, tumor necrosis factor-α in Ni-Cr and Ti alloy groups were both higher than the control group (z = 2.803, P = 0.005). However, there was no significant difference in pure Ti and Au-Pd groups compared with the control group (P > 0.05).Conclusion Pure Ti and Au-Pd alloy have a better biocompatibility than Co-Cr and Ti alloy in inflamed periodontal tissues.

Keywords

• Gingival fibroblast cell
• Co-Cr alloy
• Ti alloy
• Ti
• Au-Pd alloy