mSphere (Aug 2021)

Imaging and Quantification of mRNA Molecules at Single-Cell Resolution in the Human Fungal Pathogen Candida albicans

  • Sergio D. Moreno-Velásquez,
  • J. Christian Pérez

DOI
https://doi.org/10.1128/mSphere.00411-21
Journal volume & issue
Vol. 6, no. 4

Abstract

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Tools to visualize and quantify transcripts at single-cell resolution have enabled the dissection of spatiotemporal patterns of gene expression in animal cells and tissues. Yet the accurate quantification of transcripts at single-cell resolution remains challenging for the much smaller microbial cells.